CAJ | 학술논문

以绵羊BMPR-IB基因为候选基因,应用PCR-RFLP方法通过分析湖羊、夏洛来、陶赛特、萨福克、罗米丽、中国美利奴羊、中国美利奴肉用多胎品系以及陶赛特×中国美利奴羊和萨福克×中国美利奴羊杂交后代共615只个体的FecB基因多态性,以及BMPR-IB基因多态性对产羔数、体尺和体重的影响.结果表明,BMPR-IB基因在不同品种(系)绵羊中共有3种基因型(BB、B+和++),但基因型频率分布在各品种(系)间差异极显著(P<0.01).在湖羊中仅有BB基因型;在中国美利奴肉用多胎品系中BB、B+和++基因型频率分别为51%、30%和19%;而其他品种(系)羊中则仅有++基因型.对中国美利奴羊肉用多胎品系研究,发现BB和B+基因型群体平均产羔数分别为2.8和2.3,显著高于++基因型群体(1.2,P<0.01).在90日龄时,BB和B+基因型群体的体重分别为18.6±3.70 kg和18.0±3.31 kg,显著高于++基因型群体(15.6±2.22kg,P<0.05);此外,90日龄时,BB和B+基因型群体比++基因型群体胸围、胸宽较大(P<0.05);但这些差异在120日龄时消失.另外,我们还发现不同地区群体的第一胎产羔数存在明显差别.这些结果表明,BMPR-IB基因为影响绵羊产羔数的主效基因,并首次证明该基因对后代羔羊出生后生长发育具有加性效应.
이면양BMPR-IB기인위후선기인,응용PCR-RFLP방법통과분석호양、하락래、도새특、살복극、라미려、중국미리노양、중국미리노육용다태품계이급도새특×중국미리노양화살복극×중국미리노양잡교후대공615지개체적FecB기인다태성,이급BMPR-IB기인다태성대산고수、체척화체중적영향.결과표명,BMPR-IB기인재불동품충(계)면양중공유3충기인형(BB、B+화++),단기인형빈솔분포재각품충(계)간차이겁현저(P<0.01).재호양중부유BB기인형;재중국미리노육용다태품계중BB、B+화++기인형빈솔분별위51%、30%화19%;이기타품충(계)양중칙부유++기인형.대중국미리노양육용다태품계연구,발현BB화B+기인형군체평균산고수분별위2.8화2.3,현저고우++기인형군체(1.2,P<0.01).재90일령시,BB화B+기인형군체적체중분별위18.6±3.70 kg화18.0±3.31 kg,현저고우++기인형군체(15.6±2.22kg,P<0.05);차외,90일령시,BB화B+기인형군체비++기인형군체흉위、흉관교대(P<0.05);단저사차이재120일령시소실.령외,아문환발현불동지구군체적제일태산고수존재명현차별.저사결과표명,BMPR-IB기인위영향면양산고수적주효기인,병수차증명해기인대후대고양출생후생장발육구유가성효응.
Nine sheep breeds or strains, including 615 individuals were screened with forced PCR RFLP method for the FecB gene to study the polymorphism and its effects on litter size, body weight and body size. Results showed that the polymorphism frequencies of FecB gene were significantly imbalanced in these breeds or strains. The Hu sheep were all homozygous carriers of FecB gene(BB). In the Chinese Merino prolific meat strain, the genotype frequencies of BB, B+ and ++ were 51%, 30% and 19%, respectively, whereas all the other flocks had only the wild-type (++) genotype. Results within the Chinese Merino prolific meat strain showed that the mean litter size of ewes with genotype BB and B+ were 2.8 (±0.74) and 2.3 (±0.63) (P<0.05), whereas ++ genotype ewes had a litter size of only 1.2 (±0.68) (P<0.01). At day 90 after birth, the body weights of BB/B+ genotype lambs were higher than that of ++ genotype lambs (18.6±3.70 kg, 18.0±3.71 kg vs 15.6±2.22 kg, P<0.05). In addition, the heart girth and chest width of BB/B+ genotype lambs were significantly longer than those of the ++ lambs (P<0.05). No significant differences were observed in either body weight or body size at day 120. Litter size at first lambing from Hu at Natural Source Conservative Region was found to be significantly higher than that from the other two regions sampled (P<0.05). In addition to the additive effect on litter size, these findings showed for the first time that the FecB gene had a positive effect on early postnatal body growth.