CAJ | 학술논문

目的观察重组日本血吸虫脂肪酸结合蛋白在几种佐剂条件下的免疫预防效果.方法用E.coli表达日本血吸虫脂肪酸结合蛋白基因,制备日本血吸虫脂肪酸结合蛋白与GST的重组融合蛋白(rSj14/GST),分别以福氏完全佐剂(FCA)/福氏不完全佐剂(FIA)、卡介苗(BCG)和免疫刺激复合物(ISCOM)作为佐剂免疫昆明系小鼠,比较攻击感染后的减虫效果.结果以BCG为佐剂皮内免疫和以ISCOM为佐剂皮下免疫分别诱导了34.3%和36.0%的减虫率;而以FCA/FIA为佐剂进行免疫,虽然诱导了较高的抗体反应,但几乎没有产生明显的减虫效果(8.5%).结论 BCG和ISCOM均是rSj14/GST的适宜佐剂.
목적 관찰중조일본혈흡충지방산결합단백재궤충좌제조건하적면역예방효과.방법 용E.coli표체일본혈흡충지방산결합단백기인,제비일본혈흡충지방산결합단백여GST적중조융합단백(rSj14/GST),분별이복씨완전좌제(FCA)/복씨불완전좌제(FIA)、잡개묘(BCG)화면역자격복합물(ISCOM)작위좌제면역곤명계소서,비교공격감염후적감충효과.결과 이BCG위좌제피내면역화이ISCOM위좌제피하면역분별유도료34.3%화36.0%적감충솔;이이FCA/FIA위좌제진행면역,수연유도료교고적항체반응,단궤호몰유산생명현적감충효과(8.5%).결론 BCG화ISCOM균시rSj14/GST적괄의좌제.
Objective To test the protective immunity in mice induced by recombinant Schistosoma japonicum Sj14FABP through several adjuvant formulations. Methods The recombinant Schistosoma japonicum Sj14FABP was prepared by expression in E. coli as a GST fusion protein (rSj14/GST) and used to vaccinate outbred Kunming mice by using complete Freund's adjuvant (FCA)/incomplete Freund's adjuvant (FIA), Bacillus Calmette-Guerin (BCG) and the immunostimulating complex (ISCOM) as adjuvant respectively. Results The purified recombinant protein rSj14/GST was immunogenic in mice, and 34.3% and 36.0% worm reduction rates were obtained in outbred Kunming mice immunized intradermally with BCG adjuvant and immunized subcutaneously with ISCOM adjuvant respectively, compared with non-vaccinated control group. However, intramuscularly vaccination with rSj14/GST in FCA/FIA was not protective, although the high level of IgG antibody was induced. Conclusion Both BCG and ISCOM are suitable adjuvants for rSj14/GST.