CAJ | 학술논문

对隶属于3亚目、5次目、20科、23属共25个种类的唇口目(裸唇纲)苔藓虫18S rRNA基因部分序列进行了序列测定.结合从GenBank中获得的该类群其它7个种类的18S rRNA基因同源序列,以序列分析软件对其序列组成和变异进行了比较分析;同时,以羽苔虫(被唇纲)和管孔苔虫(窄唇纲)为外类群,以邻接法和最大简约法重建了它们的系统发生树,分析了该目主要类群系统发生关系.序列分析结果显示:经比对后序列长度为884 bp,其中保守位点241个,可变位点643个,简约信息位点357个;A,T,C和G 4碱基平均含量分别为23.8%、22.8%、24.4%和28.9%.分子系统树表明:本研究所有有囊类构成1个单系群,其中檐胞次目的几种苔虫位于皮壳次目内部;无囊类形成1个多系群,其中的亚目级(新唇口亚目)和次目级分类阶元(枝室次目、假软壁次目和隐壁次目)也都为多系发生,这些结果与前人的分子系统学研究结果大体一致,而与传统的形态分类体系间存在明显的冲突.
대대속우3아목、5차목、20과、23속공25개충류적진구목(라진강)태선충18S rRNA기인부분서렬진행료서렬측정.결합종GenBank중획득적해류군기타7개충류적18S rRNA기인동원서렬,이서렬분석연건대기서렬조성화변이진행료비교분석;동시,이우태충(피진강)화관공태충(착진강)위외류군,이린접법화최대간약법중건료타문적계통발생수,분석료해목주요류군계통발생관계.서렬분석결과현시:경비대후서렬장도위884 bp,기중보수위점241개,가변위점643개,간약신식위점357개;A,T,C화G 4감기평균함량분별위23.8%、22.8%、24.4%화28.9%.분자계통수표명:본연구소유유낭류구성1개단계군,기중첨포차목적궤충태충위우피각차목내부;무낭류형성1개다계군,기중적아목급(신진구아목)화차목급분류계원(지실차목、가연벽차목화은벽차목)야도위다계발생,저사결과여전인적분자계통학연구결과대체일치,이여전통적형태분류체계간존재명현적충돌.
The partial 18S rRNA gene sequences of 25 exarmplar species which are subordinate to 23 genera, 20 families, 5 infraorders, 3 suborders of the cheilostomatous bryozoans (class Gymnolaemata) were newly amplified and sequenced. Gombining the homologous sequences of other seven species available from GenBank, their sequence composition and variations were compared and analyzed with sequence analyzing software and their phylogenetic trees were reconstructed with neighbor joining and maximum parsimony methods using Plumatella sp. (class Phylactolaemata) and Tubulipora flabellaris (class Stenolaemata) as outgroups. The results of the sequence analyses showed that the aligned sequence is 884 bp in length, including 241 conserved, 643 variable and 357 parsimonious-informative sites, respectively; the average percentages of the content of A, T, C, G are 23.8%, 22.8%, 24.4% and 28.9%, respectivdy. The reconstructed phylogenetic trees showed that all the taxa of the suborder Ascophorina in this study is a monophyletic group, and within this group, the infraorder Umbonnlomorpha is scattered in the Lepraliomorpha; the Anasca is polyphyletic, within which the taxa of suborder Neocheilostomina and infraorders Cellulariomprpha, Pseudomalacostoegomorpha and Cryptocystoomorpha are also polyphyletic, and these results are basically the same as those of former molecular phylogenetic studies, whereas they are in conflict with those based on traditional morphological classification system.