CAJ | 학술논문

为了观察冷冻稀释液中添加不同浓度的抗氧化剂谷胱甘肽(GSH)、绿茶多酚(GTPs)、维生素E (V_E)和超氧化物歧化酶(SOD)对金华猪精液冷冻保存效果的影响,分别在冷冻稀释液中添加0.125、0.25、0.5 mmol·L～(-1)的GSH,10、15、20 mmol·L～(-1)的GTPs,1、2、4 mg·mL～(-1)的V_E和1000、2000、4000 IU的SOD,采集活率较高的金华猪精液,用上述含抗氧化剂的冷冻稀释液稀释后进行冷冻保存,分别检测4个处理组精子4 ℃平衡后的活率和解冻后精子活率、质膜完整性、顶体完整率.结果表明,在金华猪精液的冷冻稀释液中分别添加15 mmol·L～(-1) GTPs、2 mg·mL～(-1) V_E、4000 IU SOD可显著提高猪精子4 ℃平衡后的活率、解冻后精子活率和质膜完整率(P＜0.05).
위료관찰냉동희석액중첨가불동농도적항양화제곡광감태(GSH)、록다다분(GTPs)、유생소E (V_E)화초양화물기화매(SOD)대금화저정액냉동보존효과적영향,분별재냉동희석액중첨가0.125、0.25、0.5 mmol·L～(-1)적GSH,10、15、20 mmol·L～(-1)적GTPs,1、2、4 mg·mL～(-1)적V_E화1000、2000、4000 IU적SOD,채집활솔교고적금화저정액,용상술함항양화제적냉동희석액희석후진행냉동보존,분별검측4개처리조정자4 ℃평형후적활솔화해동후정자활솔、질막완정성、정체완정솔.결과표명,재금화저정액적냉동희석액중분별첨가15 mmol·L～(-1) GTPs、2 mg·mL～(-1) V_E、4000 IU SOD가현저제고저정자4 ℃평형후적활솔、해동후정자활솔화질막완정솔(P＜0.05).
To compare the effects of different antioxidant concentrations of glutathione (GSH), green tea polyphenols (GTPs), vitamin E (V_E) and superoxide dismutase (SOD) on cryopreservation of Jinhua boar sperm, freezing extenders were supplemented with the GSH (0.125, 0.25 and 0.5 mmol·L～(-1)), GTPs (10, 15 and 20 mmol·L～(-1)), V_E (1, 2 and 4 mg·mL～(-1)) and SOD (1000, 2000 and 4000 IU), respectively. The boar sperms were collected and frozen with extenders containing above antioxidants. Sperm motility after 4 ℃cooling, sperm motility, membrane integrity and acrosome integrity after thawing were examined in four treatment groups. The results show that sperm motility after 4 ℃cooling, sperm motility and membrane integrity after thawing were increased significantly by supplemented boar freezing extenders with 15 mmol·L～(-1) GTPs, 2 mg·mL～(-1) V_E or 4000 IU SOD, respectively (P< 0.05).