CAJ | 학술논문

研究了四尾栅藻(Scenedesmus quadricauda)受到不同程度缺磷胁迫后在富磷培养基中吸收磷的短期动力学过程,分析了磷在基质、细胞表面和细胞内的迁移变化过程.结果表明:经过0,30和60 h磷饥饿处理后的藻细胞,单个细胞内的磷含量分别为5.64×10~(-9),5.52×10-9和2.71×10~(-9) mg;其在富磷培养基中的最大磷吸收速率分别为0.047×10~(-9),0.098×10~(-9)和0.045×10~(-9) mg/min(以单细胞计);稳定时单个细胞内的磷含量分别为5.60×10~(-9),7.07×10~(-9)和4.20×10~(-9) mg. 经0 h饥饿处理后的藻细胞在移入富磷培养基后短时间内出现明显的内磷释放过程,经30 h饥饿处理后的藻细胞对磷的"奢侈"吸收现象明显. 此外,四尾栅藻细胞表面存在较明显的磷吸附现象,在磷充足环境中吸附量约占细胞总磷(表面吸附磷+细胞内磷)含量的1/10.
연구료사미책조(Scenedesmus quadricauda)수도불동정도결린협박후재부린배양기중흡수린적단기동역학과정,분석료린재기질、세포표면화세포내적천이변화과정.결과표명:경과0,30화60 h린기아처리후적조세포,단개세포내적린함량분별위5.64×10~(-9),5.52×10-9화2.71×10~(-9) mg;기재부린배양기중적최대린흡수속솔분별위0.047×10~(-9),0.098×10~(-9)화0.045×10~(-9) mg/min(이단세포계);은정시단개세포내적린함량분별위5.60×10~(-9),7.07×10~(-9)화4.20×10~(-9) mg. 경0 h기아처리후적조세포재이입부린배양기후단시간내출현명현적내린석방과정,경30 h기아처리후적조세포대린적"사치"흡수현상명현. 차외,사미책조세포표면존재교명현적린흡부현상,재린충족배경중흡부량약점세포총린(표면흡부린+세포내린)함량적1/10.
Experiments were carried out to study the short-term phosphorus absorption dynamics of Scenedesmus quadricauda in phosphate-replete medium after different durations of phosphorus starvation. The transferring process of phosphorus among the medium, cell surface and cell quota were analyzed. The results showed that after phosphorus starvation of 0, 30 and 60 h, the cell-quotas of phosphorus were 5.64×10~(-9), 5.52×10~(-9), and 2.71×10~(-9) mg per cell respectively. The corresponding maximum absorption rates of phosphate per cell under experimental conditions were 0.047×10~(-9), 0.098×10~(-9) and 0.045×10~(-9) mg/min. The contents of cell interior phosphorus at equilibrium were 5.60×10~(-9), 7.07×10~(-9) and 4.20×10~(-9) mg per cell separately. When moved into a phosphate-replete culture medium, algal cells starved for 0 h experienced a fast process of internal phosphate release before absorption of phosphate, while "luxury consumption" was obviously observed in algal cells starved for 30 h. Also, obvious surface-adsorption of phosphorus was observed; the amount of adsorption was about 10% of the total cell phosphorus (including surface-adsorbed and cell-quota phosphorus) in the phosphorus-replete circumstances.