CAJ | 학술논문

目的探索急性肺损伤(ALI)时肺泡Ⅱ型上皮细胞(AEC-Ⅱ)和肺表面活性蛋白A(SP-A)的变化规律.方法 110只SD幼鼠(雄性53只,雌性57只)随机分为对照组、ALI组(每组分6个亚组).ALI组腹腔注射脂多糖(LP3,4 mg/ks),对照组注射生理盐水.于注射后6,12,24,36,48,72 h每组处死8只幼鼠并取材.透射电镜下观察各组AEC-Ⅱ超微结构的变化.采用半定量RT-PCR法测量各组肺组织SP-A mRNA的表达,用Western blot法测量肺组织SP-A含量.采用SPSS 12.0统计软件包进行方差分析和方差齐性检验.结果注射LPS 24 h后,AEC-Ⅱ表面微绒毛消失.24 h和48 h时AEC-Ⅱ中板层小体(LB)出现暂时性数馈增加.48 h时LB呈特征性"指环状"绕核排列和巨大的空泡样LB.72 h时LB数目显著减少,可见破碎和残余的LB.SP-A从24 h至48 h表达显著增强(P<0.01),于36 h达最高值(6.94±0.80,P<0.01),72 h 下降至最低点(3.87 ±0.50,P<0.01).结论 ALI时AEC-Ⅱ形态变化与肺组织SP-A含量变化是时间依赖性的.AEC-Ⅱ损伤早期伴有肺组织SP-A的代偿性增加.AEC-Ⅱ损伤加重时LB出现特征性变化,可能与SP-A的代偿有关.ALI晚期,AEC-Ⅱ的严重损伤与SP-A的下降密切相关.
목적 탐색급성폐손상(ALI)시폐포Ⅱ형상피세포(AEC-Ⅱ)화폐표면활성단백A(SP-A)적변화규률.방법 110지SD유서(웅성53지,자성57지)수궤분위대조조、ALI조(매조분6개아조).ALI조복강주사지다당(LP3,4 mg/ks),대조조주사생리염수.우주사후6,12,24,36,48,72 h매조처사8지유서병취재.투사전경하관찰각조AEC-Ⅱ초미결구적변화.채용반정량RT-PCR법측량각조폐조직SP-A mRNA적표체,용Western blot법측량폐조직SP-A함량.채용SPSS 12.0통계연건포진행방차분석화방차제성검험.결과 주사LPS 24 h후,AEC-Ⅱ표면미융모소실.24 h화48 h시AEC-Ⅱ중판층소체(LB)출현잠시성수궤증가.48 h시LB정특정성"지배상"요핵배렬화거대적공포양LB.72 h시LB수목현저감소,가견파쇄화잔여적LB.SP-A종24 h지48 h표체현저증강(P<0.01),우36 h체최고치(6.94±0.80,P<0.01),72 h 하강지최저점(3.87 ±0.50,P<0.01).결론 ALI시AEC-Ⅱ형태변화여폐조직SP-A함량변화시시간의뢰성적.AEC-Ⅱ손상조기반유폐조직SP-A적대상성증가.AEC-Ⅱ손상가중시LB출현특정성변화,가능여SP-A적대상유관.ALI만기,AEC-Ⅱ적엄중손상여SP-A적하강밀절상관.
Objective To study the temporal changes of alveolar epithelial type Ⅱ cells and surfactant pro-tein A in young rats with acute lung injury induced by lipopolysaecharide. Method Totally 110 SD young rats (male:53, female : 57) were randomly divided into ALI and normal control groups (six subgroups in each group).LPS(4 mg/kg) was given intraperitoneally in ALI group. The same amount of normal saline was given in the con-trol groups. Eight rats in each subgroup were sacrificed at 6, 12, 24, 36, 48 and 72 hours after the injection.Lung samples were taken for transmission electron microscope examination. RT-PCR was epmloyed for the mea-surement of SP-A mRNA. Western blot was used for the detection of SP-A in the lung tissue. ANOVA and homo-geneity of variance test were performed by SPSS 12.0. Results The microvilli disappeared at 24 hours after the injection of LPS. The number of lamellar body (LBs) was provisionality increased at 24 hours and 48 hours. The ring-like an'angement of LBs around nucleus and the giant LB with vacuole-like deformity were found as the main characteristics of AEC- Ⅱ in ALI at 48 hours. The number of LBs reduced and broken and residual LB remained at 72 hours. SP-A elevated greatly from 24 to 48 hours (P < 0.01), reached peak at 36 hours (6.94 ± 0.80, P <0.01),reached the lowest level(3.87 ±0.50, P <0.01)at 72 hours. Conclusions The pathological changes of AEC-Ⅱ and SP-A in lung tissue wiht ALI are time-dependent. The typical alterations of AEC- Ⅱ occurs at 48 hours accompanied by the compensatory increase of SP-A. AEC- Ⅱ is seriously injuried with the typical changes of LBs and the diminishing of SP-A in lung tissue.