CAJ | 학술논문

目的探讨带蒂肌筋膜组织瓣构建组织工程喉支架形态软骨方法,为肌筋膜瓣复合组织工程化软骨修复重建喉软骨支架功能提供实验依据.方法采用溶剂浇铸、模压成形和颗粒滤沥方法制备喉支架形态聚羟基丁酸酯与聚羟基己酸酯共聚物[poly(3-hydroxybutyrate-co-3-hydroxyhexanoate),PHBHH]生物材料塑形物,接种软骨细胞形成细胞-PHBHH复合物,体外共同培养1周后用于体内植入.12只新西兰白兔,以其脊背部一侧骶棘肌及其筋膜制备肌筋膜组织瓣,采用筋膜衬里方法充填与包裹软骨细胞-PHBHH喉支架形态复合物(实验组9只),原位植入.设空白对照组(3只).分别于术后6、12和18周取材,行大体形态观察,组织学和免疫组化检测评估喉支架形态组织工程化软骨成形与再生情况.结果制备的PHBHH多孔生物材料塑形物呈中空半面喇叭状,形似喉支架形态,乙醇静态容积测定孔隙率＞90％.筋膜衬里的带蒂肌筋膜组织瓣血运丰富,可有效充填与包裹喉支架形态塑形物.不同时间点均获取大体形态维持良好的喉支架形态组织工程软骨,组织学和免疫组化检测均证实体内植入6周即可形成软骨组织,12周及18周软骨组织进一步成熟.单纯PHBHH喉支架作为对照组体内植入未检测到软骨组织.结论带蒂肌筋膜组织瓣可保障血运,采用筋膜衬里的肌筋膜组织瓣充填与包裹方法可构建喉支架形态组织工程软骨.
목적 탐토대체기근막조직판구건조직공정후지가형태연골방법,위기근막판복합조직공정화연골수복중건후연골지가공능제공실험의거.방법 채용용제요주、모압성형화과립려력방법제비후지가형태취간기정산지여취간기기산지공취물[poly(3-hydroxybutyrate-co-3-hydroxyhexanoate),PHBHH]생물재료소형물,접충연골세포형성세포-PHBHH복합물,체외공동배양1주후용우체내식입.12지신서란백토,이기척배부일측저극기급기근막제비기근막조직판,채용근막츤리방법충전여포과연골세포-PHBHH후지가형태복합물(실험조9지),원위식입.설공백대조조(3지).분별우술후6、12화18주취재,행대체형태관찰,조직학화면역조화검측평고후지가형태조직공정화연골성형여재생정황.결과 제비적PHBHH다공생물재료소형물정중공반면나팔상,형사후지가형태,을순정태용적측정공극솔＞90％.근막츤리적대체기근막조직판혈운봉부,가유효충전여포과후지가형태소형물.불동시간점균획취대체형태유지량호적후지가형태조직공정연골,조직학화면역조화검측균증실체내식입6주즉가형성연골조직,12주급18주연골조직진일보성숙.단순PHBHH후지가작위대조조체내식입미검측도연골조직.결론 대체기근막조직판가보장혈운,채용근막츤리적기근막조직판충전여포과방법가구건후지가형태조직공정연골.
Objective To explore the method of fabricating larynx-shape tissue engineered cartilage by means of filling together with wrapping with pedicle myofascial flap.Methods Serial steps of solution casting,extrusion molding and particulate leaching were used to make larynx-shape[poly(3-hydroxybutyrate-co-3-hydroxyhexanoate),PHBHH]biomaterial models.The chondrocytes were seeded onto PHBHH models to form cell-PHBHH composites for culture in vitro for one week and then to fill and wrap larynx-shape composites with pedicle myofascial flap.After that to implant larynx-shape composites in situ on the back of adult New Zealan white rabbits(experimental groups n =9).Control groups(n =3)were the same measure as experimental groups but without chondrocytes on PHBHH models.Finally,morphological observation,HE & special staining and immunohistochemical test were conducted to evaluate the cartilage regeneration and its shape at 6,8 and 12 weeks following implantation.Results The PHBHH models appeared to be hollow half-trumpet with edges and comers of larynx-shape and its porosity ＞ 90％.Pedicle myofascial flap using fascia as lining presented rich blood supply and had enough to fill and wrap larynxshape composites.Tissue engineered larynx-shape cartilage specimens could be harvested at different period.It was demonstrated that the cartilaginous tissue formed in 6 weeks after implantation through histological and immunohistochemical examination and further maturity in 12 weeks and 18 weeks.But no cartilaginous tissue showed without chondrocytes on PHBHH as control groups to implant at the same time.Conclusion It seems that pedicled myofascial flap showed sufficient blood supply and that the filling together with wrapping method with pedicled myofascial flap is appropriate for fabricating larynx-shape tissue engineered cartilage.