中国综合临床
中國綜閤臨床
중국종합림상
CLINICAL MEDICINE OF CHINA
2011年
1期
103-105
,共3页
薛慧琴%李明%朱镭%赵锐%张士勖%王薛平
薛慧琴%李明%硃鐳%趙銳%張士勗%王薛平
설혜금%리명%주뢰%조예%장사욱%왕설평
白血病%T淋巴细胞亚群%白三烯B4
白血病%T淋巴細胞亞群%白三烯B4
백혈병%T림파세포아군%백삼희B4
Acute leukemia%T lymphocyte subtype%Leukotrienes B4
目的 探讨急性白血病(AL)患儿外周血T淋巴细胞亚群和血清白三烯B4含量的变化及临床意义.方法 用免疫荧光流式细胞技术检测40例AL患儿(观察组)及40名正常儿童(对照组)外周血T淋巴细胞亚群CD3+T细胞、CD4+T细胞和CD8+T细胞水平及自然杀伤(NK)细胞活性;同时采用酶联免疫吸附试验(ELSA)方法检测上述AL患儿与正常儿童血清白三烯B4含量的变化情况.结果 AL患儿CD3+T细胞[(49.51±6.53)%]、CD4+T细胞[(22.15±4.87)%]及CD8+T细胞[(23.41±3.96)%]均低于对照组[分别为(67.63±5.23)%、(34.43±5.07)%(28.17±4.61)%],差异有统计学意义(t=13.698、t=11.047、t=4.953,P＜0.01);AL患儿自然杀伤细胞活性[(5.41±4.25)%]明显低于对照组[(13.10±4.91)%],差异有统计学意义(t=7.498,P＜0.01);而AL患儿血清白三烯B4含量[(25.23±6.24)ng/L]明显高于对照组[(8.28±2.53)ng/L],差异有统计学意义(t=15.921,P＜0.01).结论 AL患儿T淋巴细胞亚群及白三烯B4发生明显改变,对揭示AL发病机制提供重要的理论依据.
目的 探討急性白血病(AL)患兒外週血T淋巴細胞亞群和血清白三烯B4含量的變化及臨床意義.方法 用免疫熒光流式細胞技術檢測40例AL患兒(觀察組)及40名正常兒童(對照組)外週血T淋巴細胞亞群CD3+T細胞、CD4+T細胞和CD8+T細胞水平及自然殺傷(NK)細胞活性;同時採用酶聯免疫吸附試驗(ELSA)方法檢測上述AL患兒與正常兒童血清白三烯B4含量的變化情況.結果 AL患兒CD3+T細胞[(49.51±6.53)%]、CD4+T細胞[(22.15±4.87)%]及CD8+T細胞[(23.41±3.96)%]均低于對照組[分彆為(67.63±5.23)%、(34.43±5.07)%(28.17±4.61)%],差異有統計學意義(t=13.698、t=11.047、t=4.953,P＜0.01);AL患兒自然殺傷細胞活性[(5.41±4.25)%]明顯低于對照組[(13.10±4.91)%],差異有統計學意義(t=7.498,P＜0.01);而AL患兒血清白三烯B4含量[(25.23±6.24)ng/L]明顯高于對照組[(8.28±2.53)ng/L],差異有統計學意義(t=15.921,P＜0.01).結論 AL患兒T淋巴細胞亞群及白三烯B4髮生明顯改變,對揭示AL髮病機製提供重要的理論依據.
목적 탐토급성백혈병(AL)환인외주혈T림파세포아군화혈청백삼희B4함량적변화급림상의의.방법 용면역형광류식세포기술검측40례AL환인(관찰조)급40명정상인동(대조조)외주혈T림파세포아군CD3+T세포、CD4+T세포화CD8+T세포수평급자연살상(NK)세포활성;동시채용매련면역흡부시험(ELSA)방법검측상술AL환인여정상인동혈청백삼희B4함량적변화정황.결과 AL환인CD3+T세포[(49.51±6.53)%]、CD4+T세포[(22.15±4.87)%]급CD8+T세포[(23.41±3.96)%]균저우대조조[분별위(67.63±5.23)%、(34.43±5.07)%(28.17±4.61)%],차이유통계학의의(t=13.698、t=11.047、t=4.953,P＜0.01);AL환인자연살상세포활성[(5.41±4.25)%]명현저우대조조[(13.10±4.91)%],차이유통계학의의(t=7.498,P＜0.01);이AL환인혈청백삼희B4함량[(25.23±6.24)ng/L]명현고우대조조[(8.28±2.53)ng/L],차이유통계학의의(t=15.921,P＜0.01).결론 AL환인T림파세포아군급백삼희B4발생명현개변,대게시AL발병궤제제공중요적이론의거.
Objective To study the changes of T lymphocyte subtype levels and serum levels of leukotriene B4 ( LTB4 )in children with acute leukemia (AL). Methods The activity of T lymphocyte subsets:CD3+ T cells,CD4+ T cells,CD8 + T cell levels and NK cell were detected in 40 cases of AL and 40 control by Flow Cytometry(FCM). The serum level of LTB4 was measured by ELISA in all subjects. Results Compared with normal control , the percentages of CD3 + T cells ( [ 49.51 ± 6.53 ] ％ vs [ 67.63 ± 5.23 ] ％ ), CD4 + T cells ([22.15±4.87]％ vs [34.43 ±5.07]％),CD8+ T cell ([23.41 ±3.96]％ vs [28. 17 ±4.61]％),NK cell ( [5.41 ± 4.25 ] ％ vs [ 13.10 ± 4.91 ] ％ ) were significantly lower in children AL patients, ( Ps ＜0.01 ). However,the serum levels of LTB4 increased significantly in AL patients compared with normal control ( [ 25.23 ± 6.24 ] ng/L vs [ 8.28 ± 2.53 ] ng/L, P ＜ 0.01 ). Conclusion T lymphocyte subtype and serum level of LTB4 showed significant changes in children AL patients compared with normal control, which revealed an important theoretical basis to AL pathogenesis.
