CAJ | 학술논문

目的研究小檗碱对急性分离大鼠心室肌细胞内游离钙离子浓度([Ca2+]i)的影响.方法采用酶解法分离单个大鼠心肌细胞,用钙敏感的荧光指示剂Fluo-3/AM染色,以荧光强度(FI)来代表[Ca2+]i,应用激光扫描共聚焦显微镜实时监测FI的变化.结果在静息状态下,小檗碱(3～100μmol/L)对[Ca2+]i无影响.3～100μmol/L小檗碱对KCl 60mmol/L介导的钙内流也无影响.但小檗碱(30和100μmol/L)对caffeine 10mmol/L引起的钙动员有明显促进作用(P<0.01).结论小檗碱对于KCl通过电压依赖性钙通道(VDC)介导的[Ca2+]i升高无影响;但小檗碱(30和100μmol/L)可能通过影响RyRs而促进肌浆网(SR)内钙外流,也可能对SR钙摄取,钙的跨膜转运及Na+-Ca2+交换有抑制作用.
목적 연구소벽감대급성분리대서심실기세포내유리개리자농도([Ca2+]i)적영향.방법 채용매해법분리단개대서심기세포,용개민감적형광지시제Fluo-3/AM염색,이형광강도(FI)래대표[Ca2+]i,응용격광소묘공취초현미경실시감측FI적변화.결과재정식상태하,소벽감(3～100μmol/L)대[Ca2+]i무영향.3～100μmol/L소벽감대KCl 60mmol/L개도적개내류야무영향.단소벽감(30화100μmol/L)대caffeine 10mmol/L인기적개동원유명현촉진작용(P<0.01).결론소벽감대우KCl통과전압의뢰성개통도(VDC)개도적[Ca2+]i승고무영향;단소벽감(30화100μmol/L)가능통과영향RyRs이촉진기장망(SR)내개외류,야가능대SR개섭취,개적과막전운급Na+-Ca2+교환유억제작용.
Objective To investigate the effect of berberine (Ber) on intracellular calcium concentration ([Ca2+]i) in freshly isolated ventricular cells of rats.Methods An enzymatic method was used to isolate single cardiomyocytes of adult rat, which were loaded with Ca2+-sensitive fluorescent indicator Fluo-3/AM.[Ca2+]i represented by fluorescent intensity (FI) was measured by laser scanning confocal microscope (LSCM).Results At resting levels,[Ca2+]i was not affected by Ber (3～100μmol/L) in Tyrode solution containing Ca2+ 1.8mmol/L or Ca2+-free solution containing EGTA 2mmol/L. The Ca2+ influx induced by KCl 60mmol/L was not influenced by Ber (3～100μmol/L) either. Whereas Ber (30 and 100μmol/L) obviously increased Ca2+ mobilized by caffeine 10 mmol/L (P<0.01). Conclusion Ber has no effect on KCl induced [Ca2+]i elevation via voltage-dependent channel (VDC); however, Ber (30 and 100μmol/L) might promote sarcoplasmic reticulum (SR) Ca2+ efflux by affecting ryanodine receptors (RyRs), or inhibit the SR Ca2+ uptake, the transsarcolemmal Ca2+ efflux and Na+-Ca2+ exchanger.