CAJ | 학술논문

目的:探讨应用粒细胞集落刺激因子( granulocyte-colony stimulating factor,G-CSF)动员自体骨髓干细胞进入心肌梗死部位原位移植以修复梗死心肌组织,对急性心肌梗死患者心肌梗死面积及心功能的影响. 方法: 25例初次急性心肌梗死患者在入院后随机分为干细胞原位移植组( n=12)和对照组( n=13).干细胞原位移植组于入院后在常规治疗基础上加用 G-CSF 动员自体骨髓干细胞进行心肌梗死原位移植;对照组按急性心肌梗死常规方法治疗.于入院第 1,28 d描记常规 12导联心电图,采用 Wagner的 QRS波群记分法评价心功能,于入院后 7, 28 d行核素心肌灌注断层显像,测量心肌梗死面积. 结果: G-CSF治疗 4周,干细胞原位移植组 QRS记分值明显降低( 2.14± 0.21),显著低于对照组 (5.07± 0.31)( t=5.2, P< 0.05);心肌梗死面积由 (36± 8) %下降至 (18± 6) %,显著小于对照组 (33± 7)%( t=12.6, P< 0.05). 结论:用 G-CSF动员自体骨髓干细胞来修复坏死心肌组织的"干细胞原位移植"疗法,能减小心肌梗死的范围,改善心功能.
목적:탐토응용립세포집락자격인자( granulocyte-colony stimulating factor,G-CSF)동원자체골수간세포진입심기경사부위원위이식이수복경사심기조직,대급성심기경사환자심기경사면적급심공능적영향. 방법: 25례초차급성심기경사환자재입원후수궤분위간세포원위이식조( n=12)화대조조( n=13).간세포원위이식조우입원후재상규치료기출상가용 G-CSF 동원자체골수간세포진행심기경사원위이식;대조조안급성심기경사상규방법치료.우입원제 1,28 d묘기상규 12도련심전도,채용 Wagner적 QRS파군기분법평개심공능,우입원후 7, 28 d행핵소심기관주단층현상,측량심기경사면적. 결과: G-CSF치료 4주,간세포원위이식조 QRS기분치명현강저( 2.14± 0.21),현저저우대조조 (5.07± 0.31)( t=5.2, P< 0.05);심기경사면적유 (36± 8) %하강지 (18± 6) %,현저소우대조조 (33± 7)%( t=12.6, P< 0.05). 결론:용 G-CSF동원자체골수간세포래수복배사심기조직적"간세포원위이식"요법,능감소심기경사적범위,개선심공능.
AIM: To discuss the effects of self-marrow stem cells, which mobilized by the application of granulocyte-colony stimulating factor(G-CSF),on the size of infarct area and cardiac functions by its function of transplantation in situ at myocardium infarct sites for repairing infarct myocardium in acute myocardium infarction patients. METHODS: Twenty-five patients with acute myocardium infarction for the first time were randomly divided into stem cells transplantation in situ group transplantation group(n=12) and control group(n=13) after admissions. Transplantation group introduced G-CSF to mobilize self-marrow stem cells for progressing myocardium infarct site transplantation in situ except for routine therapy while the control group was treated by routine therapy after admissions.Routine 12-lead electrocardiogram was drawn on the first and the 28 th day after admissions.Wagner's QRS wave scoring system was introduced in cardiac function evaluation.Nuclein myocardium perfusion faultage image was taken on the 7th and the 28 th day after admissions to measure the infarction size. RESULTS: The QRS score values of transplantation group( 2.14± 0.21) obviously decreased compared with that of the control group(5.07± 0.31) after 4-week G-CSF therapy( t=5.2,P< 0.05) ;the infarction size decreased from(36± 8) % to(18± 6) % ,which was significantly smaller than that of the control group(33± 7) % (t=12.6,P< 0.05). CONCLUSION: The stem cell transplantation in situ therapy,which employs self-marrow stem cell that mobilize by G-CSF to repair necrosis myocardium tissue can decrease the infarction size and improve the cardiac function.