CAJ | 학술논문

建立测定人血浆中倍他米松的LC-MS/MS方法.采用Venusil XBP C8(200 mm ×3.9 mm ID,5 μm)色谱柱,流动相为甲醇-水(含甲酸铵5 mmol·L-1)(80:20),流速0.4 mL·min-1;质谱仪离子源为电喷雾离子源(ESI),正离子模式检测,监测离子为393.3→355.2(倍他米松)和361.3→343.2(泼尼松龙,内标).血浆样本用乙酸乙酯处理.倍他米松在0.5～80.0 ng·mL-1线性关系良好(r=0.999 2),血浆低、中、高3种浓度(1.0,10.0,60.0ng·mL-1)平均提取回收率为88.24%,定量限为0.5 ng·mL-1.本方法操作简便、准确、灵敏,适用于复方倍他米松注射液人体药代动力学研究.
건립측정인혈장중배타미송적LC-MS/MS방법.채용Venusil XBP C8(200 mm ×3.9 mm ID,5 μm)색보주,류동상위갑순-수(함갑산안5 mmol·L-1)(80:20),류속0.4 mL·min-1;질보의리자원위전분무리자원(ESI),정리자모식검측,감측리자위393.3→355.2(배타미송)화361.3→343.2(발니송룡,내표).혈장양본용을산을지처리.배타미송재0.5～80.0 ng·mL-1선성관계량호(r=0.999 2),혈장저、중、고3충농도(1.0,10.0,60.0ng·mL-1)평균제취회수솔위88.24%,정량한위0.5 ng·mL-1.본방법조작간편、준학、령민,괄용우복방배타미송주사액인체약대동역학연구.
A sensitive and selective liquid chromatography-tandem mass spectrometric (LC-MS/MS) method was developed and validated for the determination of betamethasone in human plasma. The analyte was isocratically eluted on a Venusil XBP C8 column (200 mm ×3.9 mm ID, 5 μm) with methanol-water with a triple quad LC-MS/MS using ESI with positive ionization. Ions monitored in the multiple reaction monitoring (MRM) mode were m/z 393.3→355.2 for betamethasone and m/z 361.3→343.2 for prednisolone (IS). Betamethasone was extracted from 0.5 mL human plasma with ethyl acetate. The validation study demonstrated excellent precision and accuracy across the calibration range of 0.5 - 80.0 injection in healthy Chinese volunteers.