CAJ | 학술논문

利用SSR分子标记技术分析了23份来自西藏不同地区的芥菜型油菜材料的遗传多样性及其地理分布的相关性.结果表明,基于遗传距离聚类法可将23份供试材料分为3大类群,品种的地理来源同聚类结果具有一定的相关性;13对SSR引物在23份供试材料中共获得58条清晰可辨的条带,其中54条具有多态性,多态性位点比率为93.1%,平均每对SSR引物检测出4.3条条带,其中3.7条条带具有多态性,表现出较高的遗传多样性;23份西藏芥菜类型油菜材料间的遗传相似系数在0.79～0.99,平均值为0.89;野生芥菜型-2与芥菜型油菜-2、野生芥菜型-5与下生分枝野生油菜之间的相似性系数最低,分别为0.79和0.80.
이용SSR분자표기기술분석료23빈래자서장불동지구적개채형유채재료적유전다양성급기지리분포적상관성.결과표명,기우유전거리취류법가장23빈공시재료분위3대류군,품충적지리래원동취류결과구유일정적상관성;13대SSR인물재23빈공시재료중공획득58조청석가변적조대,기중54조구유다태성,다태성위점비솔위93.1%,평균매대SSR인물검측출4.3조조대,기중3.7조조대구유다태성,표현출교고적유전다양성;23빈서장개채류형유채재료간적유전상사계수재0.79～0.99,평균치위0.89;야생개채형-2여개채형유채-2、야생개채형-5여하생분지야생유채지간적상사성계수최저,분별위0.79화0.80.
The genetic diversity and geographical distribution correlation of 23 B. juncea L. rapeseed materials from different areas of Tibet were analyzed by using 13 SSR molecular marker technology. The results showed that 23 test materials could be divided into 3 major groups based on genetic distance clustering method. The geographical origin of eultivars had certain relationship with the clustering results. 58 clear bands were obtained from 23 test materials by using 13 pairs of SSR primers. Among them, 54 bands was polymorphic, the ratio of polymorphic sites was 93.1 %. 4.3 bands were detected by each pair of SSR primer on the average and 3.7 bands were polymorphic with higher genetic diversity. The genetic similarity coefficient among 23 B. juncea L. rapeseed materials in Tibet ranged from 0.79 to 0.99, with the average of 0. 89. The similarity coefficients between wild B. juncea L. -2 and B. juncea L. -2, between wild B. juncea L. -5 and down-branching B. juncea L. were the lowest,being 0.79 and 0. 80 respectively.