CAJ | 학술논문

目的建立ApoE-/-小鼠腹主动脉夹层动脉瘤模型,探讨超高场强7.0T MR检测ApoE-/-小鼠腹主动脉瘤的价值. 方法 10月龄ApoE-/-小鼠饲以高脂饮食10周后,背部埋置血管紧张素Ⅱ缓释泵,Ang Ⅱ分为1000 ng/(kg·min)和500 ng/(kg·min)组,对照组埋置生理盐水,装泵前及装泵后14天内行MR活体扫描,扫描后取腹主动脉行病理学检查. 结果 Ang Ⅱ灌注高剂量组第6或第7天即形成夹层动脉瘤,T2WI示一侧管壁周可见新月形超高信号,病理证实为中膜与外膜间出血;低剂量组第13、14天后也见腹主动脉夹层动脉瘤形成,MR示管壁信号于T2WI及PDWI均明显增加,并可见斑点状高信号向腔外突出,与病理内膜断裂血流冲击中膜形成主动脉夹层一致. 结论用2种剂量的血管紧张素Ⅱ灌注2周内均可成功建立ApoE-/-小鼠腹主动脉夹层动脉瘤模型,前组可在更短时间内建模成功.超高场强MR可成功用于活体检测腹主动脉夹层动脉瘤形成.
목적 건립ApoE-/-소서복주동맥협층동맥류모형,탐토초고장강7.0T MR검측ApoE-/-소서복주동맥류적개치. 방법 10월령ApoE-/-소서사이고지음식10주후,배부매치혈관긴장소Ⅱ완석빙,Ang Ⅱ분위1000 ng/(kg·min)화500 ng/(kg·min)조,대조조매치생리염수,장빙전급장빙후14천내행MR활체소묘,소묘후취복주동맥행병이학검사. 결과 Ang Ⅱ관주고제량조제6혹제7천즉형성협층동맥류,T2WI시일측관벽주가견신월형초고신호,병리증실위중막여외막간출혈;저제량조제13、14천후야견복주동맥협층동맥류형성,MR시관벽신호우T2WI급PDWI균명현증가,병가견반점상고신호향강외돌출,여병리내막단렬혈류충격중막형성주동맥협층일치. 결론용2충제량적혈관긴장소Ⅱ관주2주내균가성공건립ApoE-/-소서복주동맥협층동맥류모형,전조가재경단시간내건모성공.초고장강MR가성공용우활체검측복주동맥협층동맥류형성.
Objective To establish abdominal aortic dissection model in ApoE-/-mice, and to evaluate the ability of 7.0T MR to detect the abdominal aortic artery aneurysms in ApoE-/-mice in vivo. Methods ApoE-/-mice aged 10 months were infused with angiotensin Ⅱ with 14 days Osmotic minipump after 10 weeks of high lipid diet. Two different doses of angiotensin Ⅱ were given to mice, i.e. 1000 ng/(kg·min) and 500 ng/(kg·min), respectively. The contrast group was infused with saline water. The abdominal aortic artery was observed in vivo with MR before and within 14 days infusion. At last, the pathological changes of the abdominal artery were compared with MRI findings. Results After 6 or 7 days higher dose of angiotensin Ⅱ infusion, aortic dissection was seen. MR T2WI showed crescent-shaped high signal in the vessel wall of one side，the pathological study identified the hematoma between media and adventitia. Abdominal aortic dissection aneurysms were also found in the mice 13 or 14 days after lower dose of angiotensin Ⅱ infusion, which were consistent with pathological studies. Besides, the signal of the vessel wall was significantly higher in both T2WI and PDWI sequences. There was excellent agreement between MR and histopathology. 〖WTHZ〗 Conclusion Abdominal aortic dissection aneurysms model can be successfully established with different doses (1000 ng/(kg·min) and 500 ng/(kg·min)) of angiotensin Ⅱ infusion into ApoE-/-mice fed with high lipid diet. High-resolution MR is able to visualize the abdominal aortic dissection aneurysm formation in vivo.