CAJ | 학술논문

目的研究地塞米松对人胸膜间皮细胞水分子通道蛋白1(AQP1)表达的影响.方法选择非感染性、良性胸腔积液患者,抽取胸水10 ml,经离心分离、获取原代胸膜间皮细胞并进行体外培养,分别以0 μmol/L、1μmol/L、100μmol/L浓度的地塞米松进行预处理后再继续进行培养,采用Western blotting及荧光定量PCR检测细胞AQP1蛋白与mRNA表达水平.结果从胸腔积液中分离与培养的人胸膜间皮细胞活细胞率和纯度均为95%以上;经地塞米松处理后的人胸膜间皮细胞AQP1与mRNA的表达水平显著增高,而且与地塞米松浓度呈正相关.结论地塞米松可以在转录水平和翻译水平上增强AQP1基因的表达,且有剂量依赖性特点.提示地塞米松可以对AQP1表达起上调作用,进而加速胸膜腔内液体的吸收.
목적 연구지새미송대인흉막간피세포수분자통도단백1(AQP1)표체적영향.방법 선택비감염성、량성흉강적액환자,추취흉수10 ml,경리심분리、획취원대흉막간피세포병진행체외배양,분별이0 μmol/L、1μmol/L、100μmol/L농도적지새미송진행예처리후재계속진행배양,채용Western blotting급형광정량PCR검측세포AQP1단백여mRNA표체수평.결과 종흉강적액중분리여배양적인흉막간피세포활세포솔화순도균위95%이상;경지새미송처리후적인흉막간피세포AQP1여mRNA적표체수평현저증고,이차여지새미송농도정정상관.결론 지새미송가이재전록수평화번역수평상증강AQP1기인적표체,차유제량의뢰성특점.제시지새미송가이대AQP1표체기상조작용,진이가속흉막강내액체적흡수.
Objective To study the effect of dexamethasone on the expression of aquaporin 1 (AQP1)in pleural mesothelial cell patients with pleural fluid. Methods Human pleural mesothelial cell was obtained from the patients with benign and non-infective pleural fluid and cultivated after preconditioning the first generation cell by 0μmol/L, 1 μmol/L, 100μmol/L dexamethasone respectively,and the expression level of AQP1 protein and mRNA was detected by western blotting and RT-PCR separately. Results The survival rate and purity of human pleural mesothelial cell isolated and cultivated from pleural fluid are both above 95 %,the expression level of AQP1 protein and mRNA of human pleural mesothelial cell induced by dexamethasone are raised up significantly. The expression level has direct correlation with the density of dexamethasone. Conclusions Dexamethasone can up-regulate AQP1 gene expression dose-dependent at translational and transcriptional level. And then accelerate the absorption of hydrothorax.