国际外科学杂志
國際外科學雜誌
국제외과학잡지
INTERNATIONAL JOURNAL OF SURGERY
2011年
9期
591-595
,共5页
张锋良%康骅%徐青%高飞%龙志华
張鋒良%康驊%徐青%高飛%龍誌華
장봉량%강화%서청%고비%룡지화
乳腺癌%17-β雌二醇%MCF-7%MRC5%基质细胞衍生因子-1(SDF-1)
乳腺癌%17-β雌二醇%MCF-7%MRC5%基質細胞衍生因子-1(SDF-1)
유선암%17-β자이순%MCF-7%MRC5%기질세포연생인자-1(SDF-1)
Breast cancer%17-β estrodial%MCF-7%MRC5%Stromal cell-derived factor-1
目的：探讨雌激素对基质成纤维细胞和乳腺癌细胞SDF-1的影响，从而揭示雌激素是否通过SDF- 1/CXCR4信号通路影响乳腺癌的生物学特性。方法 选取基质成纤维细胞MRC5和乳腺癌细胞MCF-7为研究对象，分成对照组、雌激素组和雌激素+雌激素受体阻断组，分别加入不同生理浓度的17-β雌二醇作用一定时间以及同一浓度17-β雌二醇作用不同时间点，用酶联免疫吸附试验(ELISA)方法测定培养液中SDF-1的浓度，半定量逆转录聚合酶链反应(RT- PCR)法检测细胞SDF-1 mRNA的表达。结果 MCF-7和MRC5细胞基础培养液中可检测到SDF-1分泌。不同生理浓度的17-β雌二醇均可增加细胞SDF-1的分泌水平。当加入10-7 mol/L的生理高浓度17-β雌二醇时，细胞分泌SDF-1水平在2h达到高峰，是对照组的6倍和2.7倍(P＜0.01)，该作用可被纯雌激素受体拮抗剂ICI182，780消除。此外，SDF-1 mRNA的表达水平与测得的SDF-1蛋白水平相一致，10-7 mol/L组，2h时间点SDF-1 mRNA的表达水平均高于对照组以及阻断组(P＜0.05)。结论 在基质细胞和某些乳腺癌细胞系，生理浓度的雌激素可增加SDF-1的分泌，而这种作用主要是通过雌激素受体来实现。雌激素可通过SDF-1来影响乳腺癌的生物学特性。
目的：探討雌激素對基質成纖維細胞和乳腺癌細胞SDF-1的影響，從而揭示雌激素是否通過SDF- 1/CXCR4信號通路影響乳腺癌的生物學特性。方法 選取基質成纖維細胞MRC5和乳腺癌細胞MCF-7為研究對象，分成對照組、雌激素組和雌激素+雌激素受體阻斷組，分彆加入不同生理濃度的17-β雌二醇作用一定時間以及同一濃度17-β雌二醇作用不同時間點，用酶聯免疫吸附試驗(ELISA)方法測定培養液中SDF-1的濃度，半定量逆轉錄聚閤酶鏈反應(RT- PCR)法檢測細胞SDF-1 mRNA的錶達。結果 MCF-7和MRC5細胞基礎培養液中可檢測到SDF-1分泌。不同生理濃度的17-β雌二醇均可增加細胞SDF-1的分泌水平。噹加入10-7 mol/L的生理高濃度17-β雌二醇時，細胞分泌SDF-1水平在2h達到高峰，是對照組的6倍和2.7倍(P＜0.01)，該作用可被純雌激素受體拮抗劑ICI182，780消除。此外，SDF-1 mRNA的錶達水平與測得的SDF-1蛋白水平相一緻，10-7 mol/L組，2h時間點SDF-1 mRNA的錶達水平均高于對照組以及阻斷組(P＜0.05)。結論 在基質細胞和某些乳腺癌細胞繫，生理濃度的雌激素可增加SDF-1的分泌，而這種作用主要是通過雌激素受體來實現。雌激素可通過SDF-1來影響乳腺癌的生物學特性。
목적：탐토자격소대기질성섬유세포화유선암세포SDF-1적영향，종이게시자격소시부통과SDF- 1/CXCR4신호통로영향유선암적생물학특성。방법 선취기질성섬유세포MRC5화유선암세포MCF-7위연구대상，분성대조조、자격소조화자격소+자격소수체조단조，분별가입불동생리농도적17-β자이순작용일정시간이급동일농도17-β자이순작용불동시간점，용매련면역흡부시험(ELISA)방법측정배양액중SDF-1적농도，반정량역전록취합매련반응(RT- PCR)법검측세포SDF-1 mRNA적표체。결과 MCF-7화MRC5세포기출배양액중가검측도SDF-1분비。불동생리농도적17-β자이순균가증가세포SDF-1적분비수평。당가입10-7 mol/L적생리고농도17-β자이순시，세포분비SDF-1수평재2h체도고봉，시대조조적6배화2.7배(P＜0.01)，해작용가피순자격소수체길항제ICI182，780소제。차외，SDF-1 mRNA적표체수평여측득적SDF-1단백수평상일치，10-7 mol/L조，2h시간점SDF-1 mRNA적표체수평균고우대조조이급조단조(P＜0.05)。결론 재기질세포화모사유선암세포계，생리농도적자격소가증가SDF-1적분비，이저충작용주요시통과자격소수체래실현。자격소가통과SDF-1래영향유선암적생물학특성。
Objective Stromal cell-derived factor -1 (SDF-1 ) is closely related to the biological characteristics of breast cancer. We aimed to explore whether estrogen affected breast cancer by SDF-1. Methods The breast cancer cell line MCF-7 and MRC5 were chosen, and divided into three groups: the control group, the estrogen group and the estrogen + estrogen receptor blocker group. Each group was cultured with different physiological concentrations of 17-β estrogen at certain time, and the same alcohol concentration of 17-β estradiol at different time points, and then the enzyme-linked immunosorbent assay (ELISA) was used to measure the concentration of SDF-1 in culture medium, and the semi-quantitative reverse transcriptionpolymerase chain reaction (RT -PCR) was used to detect the expression of SDF-1 mRNA in each group.Results SDF-1 can be detected in the culture medium of both MCF-7 and MRC5 cell lines. All different concentrations of 17-β estradiol may increase the secretion of SDF-1 in MCF-7 cells. When adding 17-β estradiol to the concentration of 107mol/L, the secretion of SDF-1 reached the peak in 2 hours, which was 6 times and 2.7 times that of control group ( P ＜ 0.01 ). The effect could be ehminated by pure estrogen receptor ICI182,780. In addition, the mRNA expression of SDF-1 was consistent with the SDF-1 protein levels-l07 mol/L group. The expression of SDF-1 mRNA was higher than both that of the control group and the blocking group in 2 hours (P ＜ 0.05 ). Conclusions In some breast cancer cell lines, physiological concentrations of estrogen can increase the secretion of SDF- 1, and this effect is mainly achieved through the estrogen receptor. Estrogen can influence the biological characteristics of breast cancer by SDF-1.
