CAJ | 학술논문

目的通过建立大肠癌SW620细胞裸鼠皮下移植瘤模型,观察T细胞淋巴瘤侵袭和转移诱导蛋白1( Tiam1)封闭载体在动物体内的治疗作用.方法构建编码一条短发夹RNA的Tiam1质粒表达封闭载体,并用之转染SW620细胞；应用Hoechst 33342荧光染色技术观察细胞形态学变化.将皮下接种SW620细胞的裸鼠分为3组,每组8只,在接种后第2周分别瘤内注射生理盐水(对照组)、HK错配链(HK组)和Tiam1封闭载体(Tiam1组).第16天处死各组裸鼠,取出移植瘤移重.结果通过DNA测序证实封闭载体构建成功.SW620细胞经Tiam1封闭载体转染后,细胞核可见凋亡小体.对照组平均移植瘤重量(2.84±0.27)g,HK组(2.89±0.18)g,Tiam1组(1.47±0.20)g,Tiam1组与其他2组比较差异有统计学意义(P＜0.01),而HK组和对照组之间比较差异无统计学意义(P＞0.05).结论所构建的Tiam1封闭载体能有效抑制大肠癌裸鼠皮下移植瘤生长.
목적 통과건립대장암SW620세포라서피하이식류모형,관찰T세포림파류침습화전이유도단백1( Tiam1)봉폐재체재동물체내적치료작용.방법 구건편마일조단발협RNA적Tiam1질립표체봉폐재체,병용지전염SW620세포；응용Hoechst 33342형광염색기술관찰세포형태학변화.장피하접충SW620세포적라서분위3조,매조8지,재접충후제2주분별류내주사생리염수(대조조)、HK착배련(HK조)화Tiam1봉폐재체(Tiam1조).제16천처사각조라서,취출이식류이중.결과 통과DNA측서증실봉폐재체구건성공.SW620세포경Tiam1봉폐재체전염후,세포핵가견조망소체.대조조평균이식류중량(2.84±0.27)g,HK조(2.89±0.18)g,Tiam1조(1.47±0.20)g,Tiam1조여기타2조비교차이유통계학의의(P＜0.01),이HK조화대조조지간비교차이무통계학의의(P＞0.05).결론 소구건적Tiam1봉폐재체능유효억제대장암라서피하이식류생장.
Objective To observe the tumor rate and investigate the therapeutic effect of the interference plasmid for constructed Tiam1 gene on grafted colorectal carcinoma in this nude mice model through constructed the xeno graft model of colorectal carcinoma SW620 in nude mice.Methods The RNA interference plasmid for Tiam1 gene was constructed,immmunofluorescence microscopy was used into observe the cellular morphology following Hoechst staining;the nude mice was divided to Tiam1,HK and control group,then SW620 cell was injected into the nude mice subcutaneously in the second week.The control,HK,Tiam1 plasmid were injected into the graft tumor respectively. The therapeutic effect of interference plasmid were evaluated. Results DNA sequencing confirmed the plasmid for Tiam1 gene may was successfully constructed;apoptotic body was observed in the nuclei of cells that transfected with Tiam1 shRNA plasmid under immuno-microscope.There was no difference between control and HK group (P ＞ 0.05 ).There were significant differences among Tiam1 group and other groups [ (1.47±0.20 )g vs (2.84±0.27)g (control group),(2.89±0.18)g (HK group),P＜0.01].Conclusion The interference plasmid for Tiam1 gene suppress the growth of colorectal carcinoma xeno graft in nude mice.