CAJ | 학술논문

目的分析上海地区住院腹泻患儿A组轮状病毒(RV)基因型别、流行季节及年龄分布,以了解本地区A组RV的分子流行病学动态变化特征,为婴幼儿腹泻的预防和控制提供理论依据.方法 2006年1月至2008年12月共收集来自复旦大学附属儿科医院5岁以下腹泻患儿的5176份粪便标本.抽取其中RV抗原阳性标本380份,采用套式多重RT-PCR法进行RV的基因分型.结果 2006-2008年RV腹泻主要的流行季节是10-12月份,＜3岁的患儿占全部患儿的96.8％.380份粪标本中,222份为G3型,占58.4％,41份为G9型,占10.8％.3年间G1型、G2型均少见,G混合型以G3和G9型的混合为主.2006年和2008年均以P[8]型的流行为主,分别占64.6％(53/82)和46.8％(58/124).2007年以P[4]型为主,占38.5％(67/174).P[6]和P[9]型所占比例较低,P混合型以P[8]和P[4]型的混合为主.G未分型和P未分型在3年间均呈上升趋势.P[8]G3型(20.5％)是上海地区2006-2008年间最主要的A组RV流行株,其次包括P[4]G3型及P[m]G3型.结论 2006-2008年上海地区A组RV显示了新的分子流行病学变化特征,包括主要流行型别的改变,流行型别更趋于多样化.
목적 분석상해지구주원복사환인A조륜상병독(RV)기인형별、류행계절급년령분포,이료해본지구A조RV적분자류행병학동태변화특정,위영유인복사적예방화공제제공이론의거.방법 2006년1월지2008년12월공수집래자복단대학부속인과의원5세이하복사환인적5176빈분편표본.추취기중RV항원양성표본380빈,채용투식다중RT-PCR법진행RV적기인분형.결과 2006-2008년RV복사주요적류행계절시10-12월빈,＜3세적환인점전부환인적96.8％.380빈분표본중,222빈위G3형,점58.4％,41빈위G9형,점10.8％.3년간G1형、G2형균소견,G혼합형이G3화G9형적혼합위주.2006년화2008년균이P[8]형적류행위주,분별점64.6％(53/82)화46.8％(58/124).2007년이P[4]형위주,점38.5％(67/174).P[6]화P[9]형소점비례교저,P혼합형이P[8]화P[4]형적혼합위주.G미분형화P미분형재3년간균정상승추세.P[8]G3형(20.5％)시상해지구2006-2008년간최주요적A조RV류행주,기차포괄P[4]G3형급P[m]G3형.결론 2006-2008년상해지구A조RV현시료신적분자류행병학변화특정,포괄주요류행형별적개변,류행형별경추우다양화.
Objective To investigate the dynamic characteristic of molecular epidemiology of group A Rotavirus (RV) by analyzing viral genotypes,disease seasonality,and the patients' age distribution,so that to provide theoretical basis for preyention and control of RV diarrhea in children.Methods A total of 380 RV antigen positive samples were selected from 5176 stool specimens collected from ＜5 year-old patients with acute diarrhea who were admitted to Children's Hospital of Fudan University during January 2006 to December 2008. Multiplex nested reverse transcription polymerase chain reaction (RT-PCR) was used to analyze the RV genotypes.Results During 2006-2008,the incidence of RV related diarrhea peaked from October to December and about 96.8％ of all RV episodes occurred in patients younger than 3 years old,The predominant genotype was G3 which accounted for 58.4％ (222/380),G9 was an emerging genotype with the prevalence rate as high as 10.8％ (41/380).G1 and G2 types were rarely found during the three years.Infections with both G3 and G9 were the major mixed genotype G infection. Genotype P [8] was predominant with the prevalence rates of 64.6％ (53/82) and 46.8％ (58/124) in 2006 and 2008,respectively,whereasgenotype P[4] was predominant in 2007 (38,5％,67/174).P[6] and P[9] were found as minor types.The major mixed genotype P infection were genotype P[4] and P[8]. The proportion of undetermined genotype G and genotype P strains tended to increasing during 2006-2008.Genotype P [8]G3 was the major RV strain (20.5％) in Shanghai during 2006-2008 and the other prevalent genotypes included P[4]G3 and P[m]G3.Conclusion The infection of group A RV in Shanghai presents some new molecular epidemiology characteristics during 2006-2008,such as switch of predominant genotypes and diversification of prevalent genotypes.