CAJ | 학술논문

以台盼蓝染色方法观察了大鼠胚胎脑组织在五种保存液中的保存效果,初步结果提示无血清DMEM培养液是一种较好的胎脑组织保存液,保存胎脑组织5天时平均细胞存活率达70%以上.将于DMEM液中保存5天的胎鼠大脑组织植入同种大鼠脑内,6～8周后进行组织学观察,结果发现移植物有存活,但其存活质量明显不如新鲜组织移植.
이태반람염색방법관찰료대서배태뇌조직재오충보존액중적보존효과,초보결과제시무혈청DMEM배양액시일충교호적태뇌조직보존액,보존태뇌조직5천시평균세포존활솔체70%이상.장우DMEM액중보존5천적태서대뇌조직식입동충대서뇌내,6～8주후진행조직학관찰,결과발현이식물유존활,단기존활질량명현불여신선조직이식.
Five kinds of solutions,i.e.NS,Freds,Euro-Collins,CSF and DMEM,were used to preserve fetal rat brain tissue at 4℃.Cell survival rates were monitored by trypan blue test.Preliminary results indicate DMEM has a good effect.More than 70% of brain cells survived at the fifth day.Fresh(A)and 5 day preserved(B) cerebral tissues were grafted into the cortex of adult male rats.Histological studies carried out 6～8 weeks later showed graft survival.much better survival quality in fresh graft tissue group was observed.