CAJ | 학술논문

目的探讨 Fas相关死亡域蛋白( FADD)在增生性瘢痕( HS)各个时期的表达变化以及与 HS演变的关系.方法采用原位杂交与图象分析系统相结合的方法,定量观察 FADD(2份,取自同一部位样本 )在 HS增殖期、成熟期和消退期的表达分布及表达量.结果 FADD在 HS成熟期的表达量 [面度( 9.36± 0.92),平均光密度( 1154± 68)〗是增殖期 [面度( 4.72± 0.35),平均光密度( 552± 32)〗的 2倍,是消退期 [面度( 1.88± 0.12),平均光密度( 218± 16)〗的 5倍; FADD阳性信号主要见于表皮棘层、真皮网状层,以及部分血管.结论 FADD表达与增生性瘢痕演变密切相关.
목적탐토 Fas상관사망역단백( FADD)재증생성반흔( HS)각개시기적표체변화이급여 HS연변적관계.방법채용원위잡교여도상분석계통상결합적방법,정량관찰 FADD(2빈,취자동일부위양본 )재 HS증식기、성숙기화소퇴기적표체분포급표체량.결과 FADD재 HS성숙기적표체량 [면도( 9.36± 0.92),평균광밀도( 1154± 68)〗시증식기 [면도( 4.72± 0.35),평균광밀도( 552± 32)〗적 2배,시소퇴기 [면도( 1.88± 0.12),평균광밀도( 218± 16)〗적 5배; FADD양성신호주요견우표피극층、진피망상층,이급부분혈관.결론 FADD표체여증생성반흔연변밀절상관.
Aim To explore the change of in situ expression of FADD (Fas protein associated with death domain) at various stages in hypertrophic scars and its implication.Methods The change of expression of FADD genes at mature stage, proliferative stage and regressive stages in HS was quantitatively investigated by in situ hybridization techniques and imagine analysis system.Results Expressive intention of FADD genes at the mature stage[area density( 9.36± 0.92), average integral optical density( 1154± 68)〗 was 2 times as that at the proliferative stage[area density( 4.72± 0.35), average integral optical density( 552± 32)〗 ,and 5 times as that at regressive stage[area density( 1.88± 0.12), average integral optical density( 218± 16)〗 .Staining sites located in the stratum spinosum of epidermis,reticular dermis and part vessels.Conclusion FADD expression is tightly associated with the development of hypertrophic scars.