CAJ | 학술논문

目的: 制备呋甾皂苷的生物转化产物.方法:利用米曲霉对原薯蓣皂苷(化合物1)进行转化,转化产物用大孔吸附树脂柱色谱,C18反相硅胶柱色谱,硅胶柱色谱以及高效液相色谱等进行分离纯化,通过光谱分析(FAB-MS,1H NMR,13C NMR)鉴定化学结构.结果:得到了5个转化产物,包括3个呋甾皂苷和2个螺甾皂苷.结论:米曲霉可以在保留原薯蓣皂苷的26-位葡萄糖的情况下水解3-位末端1→2鼠李糖生成次生呋甾皂苷(化合物4).米曲霉也可以去掉原薯蓣皂苷的22-位羟基生成20(22)双键的呋甾皂苷产物(化合物3和5),并且继续水解26-位葡萄糖生成相应的螺甾皂苷(化合物2和6).
목적: 제비부치조감적생물전화산물.방법:이용미곡매대원서여조감(화합물1)진행전화,전화산물용대공흡부수지주색보,C18반상규효주색보,규효주색보이급고효액상색보등진행분리순화,통과광보분석(FAB-MS,1H NMR,13C NMR)감정화학결구.결과:득도료5개전화산물,포괄3개부치조감화2개라치조감.결론:미곡매가이재보류원서여조감적26-위포도당적정황하수해3-위말단1→2서리당생성차생부치조감(화합물4).미곡매야가이거도원서여조감적22-위간기생성20(22)쌍건적부치조감산물(화합물3화5),병차계속수해26-위포도당생성상응적라치조감(화합물2화6).
AIM: To prepare biotransformation products of furostanol saponin. METHOD: Protodioscin (compound 1) was biotransformed by Aspergillus oryzae and the products were purified by column chromatography of macroporous absorbent resin and reverse phase C18 and high performance liquid chromatography. The structures of products were ascertained by FAB-MS, 1H NMR and 13C NMR. RESULT: Biotransformation of protodioscin led to five main bioconversion products including three furostanol saponins and two spirostanol saponins. CONCLUSION: It has been found that Aspergillus oryzae is able to hydrolyze the terminal rhamnopyranosyl residue with 1→2 linkage to sugar chain of protodioscin at C-3 position, while retaining glucopyranosyl residue at C-26 position to produce secondary furostanol saponin (compound 4). Aspergillus oryzae also can abscise the hydroxyl at C-22 position of furostanol saponins to produce furostanol saponins (compounds 3 and 5), which have ethylenic linkage between C-20 and C-22 position, and hydrolyze the glucopyranosyl residue of furostanol saponins at C-26 position to produce corresponding spirostanol saponins (compounds 2 and 6).