CAJ | 학술논문

目的探讨wt-P53蛋白对人瘢痕疙瘩成纤维细胞(keloid fibroblasts,KFBs)端粒酶活性的影响;明确在人KFBs中wt-P53蛋白与端粒酶活性之间的相互关系.方法将来源于人瘢痕疙瘩组织的KFBs随机分成两组,转染组采用腺病毒介导法将野生型wt-p53基因转染至人KFBs;非转染组KFBs未进行野生型wt-p53基因转染.转染48 h后,采用间接免疫荧光法和Western blotting法检测KFBs wt-P53蛋白的表达;并于转染后1～7 d,采用TRAP-ELISA法检测KFBs端粒酶活性.结果两组均有wt-P53蛋白表达,转染组wt-P53蛋白表达明显高于非转染组;转染后1～7 d,转染组端粒酶活性均明显低于非转染组(P＜0.05).结论 wt-P53蛋白能够抑制人KFBs端粒酶活性.
목적 탐토wt-P53단백대인반흔흘탑성섬유세포(keloid fibroblasts,KFBs)단립매활성적영향;명학재인KFBs중wt-P53단백여단립매활성지간적상호관계.방법 장래원우인반흔흘탑조직적KFBs수궤분성량조,전염조채용선병독개도법장야생형wt-p53기인전염지인KFBs;비전염조KFBs미진행야생형wt-p53기인전염.전염48 h후,채용간접면역형광법화Western blotting법검측KFBs wt-P53단백적표체;병우전염후1～7 d,채용TRAP-ELISA법검측KFBs단립매활성.결과 량조균유wt-P53단백표체,전염조wt-P53단백표체명현고우비전염조;전염후1～7 d,전염조단립매활성균명현저우비전염조(P＜0.05).결론 wt-P53단백능구억제인KFBs단립매활성.
Objective To evaluated the role of wt-P53 protein in telomerase regulation in keloid fibroblasts(KFBs). Methods The fibroblasts were derived from human keloid tissue which was proved by pathological diagnosis. KFBs were divided into 2 groups, the transfection group and the untransfection group. wt-p53 gene was transfected into the fibroblasts by adenovirus vectors in the transfection group. The KFBs untransfected with wt-p53 gene served as control (untransfection group). After 48 hours of transfection, the expression of wt-P53 protein was analyzed by both Western blotting and immunofluorescence method, respectively. The telomerase activity was evaluated by TRAP-ELISA after 1-7 days of transfection.Results All the KFBs from 2 groups expressed wt-P53 protein. But the expression level of wt-P53 protein in the transfection group was significantly higher than that in the untransfection group. At the same time of high expression of wt-P53 protein, the telomerase activity of KFBs in transfection group was significantly lower than that in the untransfection group( P＜0.05). Conclusion High level expression of wt-P53 protein can transiently inhibit the telomerase activity of KFBs.