CAJ | 학술논문

目的:研究内脏高敏感大鼠的脊髓、脑部CRF的分布和表达,探讨CRF在肠易激综合征(IBS)内脏高敏感信号传导通路中的机制,以及肠炎宁糖浆对IBS起效的可能作用机制.方法:清洁级成年♀SD大鼠40只,随机分3组,空白组n=8),模型一组(腹腔注射鸡卵清蛋白致敏,n=16),模型二组(条件刺激和非条件刺激轮替致敏,n=16),评估肠道敏感性后,模型一组和模型二组均随机分成2组,即对照组和肠炎宁组,每组8只.空白组和对照组给予生理盐水,肠炎宁组给予肠炎宁,ig4 wk,取脑、脊髓进行免疫组化,观察CRF的分布和表达情况.结果:肠炎宁组内脏敏感性较模型组明显降低(P<0.01).免疫组化显示大鼠下丘脑、第三脑室下侧、脊髓腰膨大部可见CRF的明显表达, CRF阳性指数模型一对照组和模型二对照组均高于空白对照组,差异有统计学意义邋(下丘脑:0.037±0.009,0.037±0.024 vs 0.005±0.001:第三脑室下侧:0.038±0.009,0.040±0.022 vs 0.005±0.00 1:脊髓:0.028 + 0.008,0.024±0.004 vs 0.002±0.001; 均P<0.01).模型一肠炎宁组(0.012±0.005,0.012±0.005,0.010±0.003)较模型一对照组高, 模型二肠炎宁组(0.009±0.005,0.011±0.006,0.012±0.005)较模型二对照组高,模型一肠炎宁组和模型二肠炎宁组较空白对照组高,差异均有统计学意义CP<0.05).结论:CRF在内脏刺激信号的传入过程中起重要作用,肠炎宁可以降低其表达程度,这可能是降低大鼠内脏高敏感的机制之一.
목적:연구내장고민감대서적척수、뇌부CRF적분포화표체,탐토CRF재장역격종합정(IBS)내장고민감신호전도통로중적궤제,이급장염저당장대IBS기효적가능작용궤제.방법:청길급성년♀SD대서40지,수궤분3조,공백조n=8),모형일조(복강주사계란청단백치민,n=16),모형이조(조건자격화비조건자격륜체치민,n=16),평고장도민감성후,모형일조화모형이조균수궤분성2조,즉대조조화장염저조,매조8지.공백조화대조조급여생리염수,장염저조급여장염저,ig4 wk,취뇌、척수진행면역조화,관찰CRF적분포화표체정황.결과:장염저조내장민감성교모형조명현강저(P<0.01).면역조화현시대서하구뇌、제삼뇌실하측、척수요팽대부가견CRF적명현표체, CRF양성지수모형일대조조화모형이대조조균고우공백대조조,차이유통계학의의랍(하구뇌:0.037±0.009,0.037±0.024 vs 0.005±0.001:제삼뇌실하측:0.038±0.009,0.040±0.022 vs 0.005±0.00 1:척수:0.028 + 0.008,0.024±0.004 vs 0.002±0.001; 균P<0.01).모형일장염저조(0.012±0.005,0.012±0.005,0.010±0.003)교모형일대조조고, 모형이장염저조(0.009±0.005,0.011±0.006,0.012±0.005)교모형이대조조고,모형일장염저조화모형이장염저조교공백대조조고,차이균유통계학의의CP<0.05).결론:CRF재내장자격신호적전입과정중기중요작용,장염저가이강저기표체정도,저가능시강저대서내장고민감적궤제지일.