CAJ | 학술논문

目的探讨雷洛昔芬对大鼠急性肺损伤(acute lung injury,ALI)的保护作用.方法 30只SD雄性大鼠按随字数字表法分为三组:二次打击前用药组(10只)、二次打击中用药组(10只)和对照组(10只).所有大鼠腹腔注射5 mg/kg LPS,并分别于LPS腹腔注射前1 h和腹腔注射后14 h对二次打击前用药组和二次打击中用药组用雷洛昔芬30 mg/kg灌胃,LPS腹腔注射后16 h,所有大鼠均用戊巴比妥钠按40 mg/kg行腹腔注射麻醉,股动脉插管监测平均动脉压(MAP),气管内滴入pH为1.2,0.5 ml/kg盐酸.盐酸滴入前及滴入后30,90 min和4 h查动脉血气分析,4 h后每组中选取5只大鼠行[18F]FDG microPET胸部扫描,而后取肺组织进行组织病理学观察.结果血气分析显示二次打击中用药组大鼠能显著改善肺的氧合功能,并能使MAP稳定,[18F]FDG吸收度及肺组织病理评分在对照组分别为9.01±1.58,12.6±0.97,显著高于二次打击中用药组的4.67±1.33(P<0.01)和8.20±1.23(P<0.01).结论雷洛昔芬对大鼠ALI具有明显保护作用;[18F]FDG microPET能很好地评价ALI时肺内的炎症反应.
목적 탐토뢰락석분대대서급성폐손상(acute lung injury,ALI)적보호작용.방법 30지SD웅성대서안수자수자표법분위삼조:이차타격전용약조(10지)、이차타격중용약조(10지)화대조조(10지).소유대서복강주사5 mg/kg LPS,병분별우LPS복강주사전1 h화복강주사후14 h대이차타격전용약조화이차타격중용약조용뢰락석분30 mg/kg관위,LPS복강주사후16 h,소유대서균용무파비타납안40 mg/kg행복강주사마취,고동맥삽관감측평균동맥압(MAP),기관내적입pH위1.2,0.5 ml/kg염산.염산적입전급적입후30,90 min화4 h사동맥혈기분석,4 h후매조중선취5지대서행[18F]FDG microPET흉부소묘,이후취폐조직진행조직병이학관찰.결과 혈기분석현시이차타격중용약조대서능현저개선폐적양합공능,병능사MAP은정,[18F]FDG흡수도급폐조직병리평분재대조조분별위9.01±1.58,12.6±0.97,현저고우이차타격중용약조적4.67±1.33(P<0.01)화8.20±1.23(P<0.01).결론 뢰락석분대대서ALI구유명현보호작용;[18F]FDG microPET능흔호지평개ALI시폐내적염증반응.
Objective To evalhate the protective effect of oral raloxifene on lung function after acute lung injury (ALI) in rats. Methods Thirty male adult Sprague-Dawley rats were used and divided into three groups: LPS raloxifene hydrochloric acid. group before secondary impact ( Group A, n = 10 ), LPS raloxifene hydrochloric acid group after secondary impact ( Group B, n = 10) and control group ( n = 10). All the rats were injected intraperitoneally with 5 mg/kg LPS. Raloxifene (30 mg/kg) was orally administered one hour before LPS injection and 14 hours after LPS injection in Groups A and B. The con-trol group remained free. All the animals were anesthetized by intraperitoneal injection of pentobarbital so-dium at 40 mg/kg and the femoral artery was cannulated 16 hours after LPS injection to measure the mean arterial pressure (MAP). All the rats received a direct intratracheal injection of hydrochloric acid ( pH = 1.2, 0.5 ml/kg). Before injection of hydrochloric acid and at 0. 5,1.5 and 4 hours after injection of hy-drochloric acid, the blood gas was measured. Fifteen rats ( five from each group) underwent a micro posi-tron emission tomography ( [18F] FDG microPET) scan of the thorax four hours after hydrochloric acid in-stillation. Then, the lung tissue was collected for histopathological examination. Results The Group B showed better pulmonary gas exchange and more stable MAP compared to the control group. The [18F] fluorodeoxyglueose uptake and histological lung injury score were 9. 01 ± 1.58 and 12.6 ± 0.97 respec-tively in Group B, which were higher than 4. 67 ± 1.33 and 9. 01 ± 1.58 respectively in control group (P < 0. 01 ). Conclusions Raloxifene exerts significant protective effect on lung function after ALI. [18F] FDG microPET is a useful method to evaluate the inflammatory reaction during ALI.