CAJ | 학술논문

棉铃虫Helicoverpa armigera是一种严重危害棉花等经济作物的鳞翅目害虫,开展分子水平研究对防控害虫将具有重要参考意义.本研究利用RACE(rapid amplification of cDNA ends)技术克隆了棉铃虫磷酸甘油醛异构酶(triosephosphate isomerase)基因Hatpi(GenBank登录号为AY736358).该基因cDNA全长为1 149 bp,编码248个氨基酸,预测等电点为5.82,分子量为16.4 kD.HaTPI含有磷酸甘油醛异构酶类蛋白的典型(βα)_8结构、保守的活性位点(Lys12,His94和Glu165)和小肽序列(AYEPVWAIGTG和GGASLKPEF)等.RT-PCR检测分析发现Hatpi在棉铃虫卵巢、幼虫、蛹、成虫均有表达,提示该基因可能在棉铃虫的不同发育阶段均起作用.
면령충Helicoverpa armigera시일충엄중위해면화등경제작물적린시목해충,개전분자수평연구대방공해충장구유중요삼고의의.본연구이용RACE(rapid amplification of cDNA ends)기술극륭료면령충린산감유철이구매(triosephosphate isomerase)기인Hatpi(GenBank등록호위AY736358).해기인cDNA전장위1 149 bp,편마248개안기산,예측등전점위5.82,분자량위16.4 kD.HaTPI함유린산감유철이구매류단백적전형(βα)_8결구、보수적활성위점(Lys12,His94화Glu165)화소태서렬(AYEPVWAIGTG화GGASLKPEF)등.RT-PCR검측분석발현Hatpi재면령충란소、유충、용、성충균유표체,제시해기인가능재면령충적불동발육계단균기작용.
Helicoverpa armigera is an important lepidopteran pest of many crops including cotton. Molecular analysis will promote pest control in the future. The gene that encoding triosephosphate isomerase in Helicoverpa armigera, Hatpi, was molecularly cloned and analyzed in this study. The cDNA of Hatpi (GenBank accession no. AY736358) is 1 149 bp in length and encodes a 248 amino acid protein with the estimated molecular mass of 26.4 kD and isoelectric point of 5.82. (βα)_8 structure found in the deduced protein structure of HaTPI was similar to that of other triosephosphate isomerase. High identity was also found in the active catalytic sites (Lys12, His94, and Glu165) and peptide motifs (AYEPVWAIGTG and GGASLKPEF). RT-PCR analysis results showed that Hatpi was expressed in the embryo, larva, pupa and adult of H. armigera, suggesting Hatpi may play roles in various developmental stages of H. armigera.