CAJ | 학술논문

目的探讨异丙酚对小鼠脑动脉血管平滑肌细胞自发性瞬时外向钾电流的影响.方法昆明小鼠,体重18～22 g,雌雄不拘,分离脑底部Willis动脉环,经两步法酶消化分离血管平滑肌细胞,选取5个血管平滑肌细胞,采用穿孔全细胞膜片钳技术,于钳制电位为- 30 mV时记录加入56μmol/L异丙酚前后自发性瞬时外向钾电流,分析其幅度、频率、曲线下面积和时间半宽.结果与给药前比较,给药后自发性瞬时外向钾电流的幅度、频率和曲线下面积升高(P＜0.05或0.01),时间半宽差异无统计学意义(P＞0.05).结论异丙酚可激活小鼠脑动脉血管平滑肌细胞自发性瞬时外向钾电流,诱发血管平滑肌舒张.
목적 탐토이병분대소서뇌동맥혈관평활기세포자발성순시외향갑전류적영향.방법 곤명소서,체중18～22 g,자웅불구,분리뇌저부Willis동맥배,경량보법매소화분리혈관평활기세포,선취5개혈관평활기세포,채용천공전세포막편겸기술,우겸제전위위- 30 mV시기록가입56μmol/L이병분전후자발성순시외향갑전류,분석기폭도、빈솔、곡선하면적화시간반관.결과 여급약전비교,급약후자발성순시외향갑전류적폭도、빈솔화곡선하면적승고(P＜0.05혹0.01),시간반관차이무통계학의의(P＞0.05).결론 이병분가격활소서뇌동맥혈관평활기세포자발성순시외향갑전류,유발혈관평활기서장.
Objective To investigate the effects of propofol on the spontaneous transient outward K+ currents in mouse cerebral arterial smooth muscle cells.Methods Kunming mice of both sexes,weighing 18-22 g,were used in this study.Vascular smooth muscle cells were freshly isolated from cerebral arteries in two steps.Five cells were chosen and studied.When holding potential was - 30 mV,spontaneous transient outward K+ currents were recorded before and after the application of 56 μmol/L propofol by perforated whole-cell patch-clamp technique.The amplitude,frequency,area under the curve and half time width of spontaneous transient outward K+ currents were analyzed.Results Propofol 56 μmol/L significantly increased the amplitude,frequency and area under the curve of spontaneous transient outward K+ currents.There was no significant change in the half time width of spontaneous transient outward K+ currents after administration,Conclusion Propofol can activate spontaneous transient outward K+ currents in mouse cerebral arterial smooth muscle cells,and thus induces vascular smooth muscle relaxation.