中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2010年
12期
1437-1439
,共3页
脑啡肽类%生物,基因修饰%干细胞%注射,脊髓%神经痛
腦啡肽類%生物,基因脩飾%榦細胞%註射,脊髓%神經痛
뇌배태류%생물,기인수식%간세포%주사,척수%신경통
Enkephalins%Organisms,genetically modified%Stem cells%Injections,spinal%Neuralgia
目的 探讨神经病理性痛大鼠鞘内注射人前脑啡肽原(PENK)基因修饰人骨髓间充质干细胞(hMSC)的镇痛效果.方法 取鞘内置管成功的健康雄性SD大鼠40只,周龄6～8周,体重160～180 g,随机分为4组(n=10),A组为正常对照组;B组、C组和D组采用坐骨神经慢性压迫性损伤(CCI)法建立神经病理性痛模型,于CCI术后3 d鞘内给药,A组和B组鞘内注射生理盐水10μl;C组鞘内注射转染空载体的hMSC细胞(hMSC-pBABE)悬液10μl(2×108～3×108/μl);D组鞘内注射hMSC-PENK细胞悬μl液10μl(2×108～3×108个/μl).于术前、术后3、5、7、9、14 d时测定热痛阈.术后14 d痛阈测定后取新鲜脊髓组织,采用RT-PCR法测定PENK mRNA表达.结果 与术前比较,B组、C组、D组术后各时点热痛阈降低(P＜0.05).与A组比较,B组、C组、D组热痛阈降低,B组和C组PENK mRNA表达下调,D组PENK mRNA表达上调(P＜0.05).与B组和C组比较,D组热痛阈升高,PENK mRNA表达上调(P＜0.05).B组和C组各指标比较差异无统计学意义(P＞0.05).结论 大鼠鞘内注射PENK基因修饰的hMSC可减轻神经病理性痛.
目的 探討神經病理性痛大鼠鞘內註射人前腦啡肽原(PENK)基因脩飾人骨髓間充質榦細胞(hMSC)的鎮痛效果.方法 取鞘內置管成功的健康雄性SD大鼠40隻,週齡6～8週,體重160～180 g,隨機分為4組(n=10),A組為正常對照組;B組、C組和D組採用坐骨神經慢性壓迫性損傷(CCI)法建立神經病理性痛模型,于CCI術後3 d鞘內給藥,A組和B組鞘內註射生理鹽水10μl;C組鞘內註射轉染空載體的hMSC細胞(hMSC-pBABE)懸液10μl(2×108～3×108/μl);D組鞘內註射hMSC-PENK細胞懸μl液10μl(2×108～3×108箇/μl).于術前、術後3、5、7、9、14 d時測定熱痛閾.術後14 d痛閾測定後取新鮮脊髓組織,採用RT-PCR法測定PENK mRNA錶達.結果 與術前比較,B組、C組、D組術後各時點熱痛閾降低(P＜0.05).與A組比較,B組、C組、D組熱痛閾降低,B組和C組PENK mRNA錶達下調,D組PENK mRNA錶達上調(P＜0.05).與B組和C組比較,D組熱痛閾升高,PENK mRNA錶達上調(P＜0.05).B組和C組各指標比較差異無統計學意義(P＞0.05).結論 大鼠鞘內註射PENK基因脩飾的hMSC可減輕神經病理性痛.
목적 탐토신경병이성통대서초내주사인전뇌배태원(PENK)기인수식인골수간충질간세포(hMSC)적진통효과.방법 취초내치관성공적건강웅성SD대서40지,주령6～8주,체중160～180 g,수궤분위4조(n=10),A조위정상대조조;B조、C조화D조채용좌골신경만성압박성손상(CCI)법건립신경병이성통모형,우CCI술후3 d초내급약,A조화B조초내주사생리염수10μl;C조초내주사전염공재체적hMSC세포(hMSC-pBABE)현액10μl(2×108～3×108/μl);D조초내주사hMSC-PENK세포현μl액10μl(2×108～3×108개/μl).우술전、술후3、5、7、9、14 d시측정열통역.술후14 d통역측정후취신선척수조직,채용RT-PCR법측정PENK mRNA표체.결과 여술전비교,B조、C조、D조술후각시점열통역강저(P＜0.05).여A조비교,B조、C조、D조열통역강저,B조화C조PENK mRNA표체하조,D조PENK mRNA표체상조(P＜0.05).여B조화C조비교,D조열통역승고,PENK mRNA표체상조(P＜0.05).B조화C조각지표비교차이무통계학의의(P＞0.05).결론 대서초내주사PENK기인수식적hMSC가감경신경병이성통.
Objective To investigate the analgesic effect of intrathecal(IT)human bone marrow mesenchymal stem cells(hMSC)genetically modified with human proenkephalin gene(PENK)in a rat model of neuropathic pain.Methods Forty male SD rats weighing 160-180 g in which IT catheters were successfully implanted without complication were randomly divided into 4 gorups(n = 10 each): group A normal control;group B neuropathic pain(NP);group C NP + hMSC-pBABE and group D NP + hMSC-PENK.Neuropathic pain was induced with chronic constrictive injury(CCI).Four loose ligatures were placed on the main stem of sciatic nerve with 4-0 chronic catgut.IT normal saline 10 μl,hMSC-pBABE cell suspension 10 μl(2 × 108-3 × 108/μl)and hMSCPENK cell suspension 10 μl(2 × 108-3 × 108/μl)were injected in group B,C and D respectively on the 3rd day after operation.Paw-withdrawal latency(PWL)to noxious thermal stimulation was measured before(baseline)and at 3,5,7,9 and 14 d after operation.The animals were killed on the 14th day after last PWL measurement.RNA was extracted from the spinal cord for determination of proenkephalin mRNA expression.Results PWL was significantly decreased after operation as compared with the baseline values before operation in group B,C and D.PWL was significantly longer at 7,9,14 d after operation in group D than in group B and C but there was no significant difference in PWL after operation between group B and C.PENK mRNA expression was significantly lower in group B and C than in group A,but was significantly higher in group D than in group B and C.There was no significant difference in PENK mRNA expression between group B and C.Conclusion Intratheccal human bone marrow mesenchymal stem cells genetically modified with human proenkephalin gene can relieve neuropathic pain in rats.
