华北农学报
華北農學報
화북농학보
ACTA AGRICULTURAE BOREALI-SINICA
2009年
5期
7-10
,共4页
常惠芸%丛国正%独军政%邵军军%林彤%冯金瑞%刘萍
常惠蕓%叢國正%獨軍政%邵軍軍%林彤%馮金瑞%劉萍
상혜예%총국정%독군정%소군군%림동%풍금서%류평
C型口蹄疫病毒%重组蛋白P1%表达生物活性
C型口蹄疫病毒%重組蛋白P1%錶達生物活性
C형구제역병독%중조단백P1%표체생물활성
FMDV type C%Recombiant P1%Expression biology acting
将口蹄疫病毒(FMDV)结构蛋白基因P1的完整cDNA序列插入原核表达性载体pET-28α(+)中,获得融合表达质粒pET-P1,转化E.coli.BL21(DE3),经IPTG诱导,SDS-PAGE结果表明,pET-P1获得融合表达, Western Blot 检测证实表达的融合蛋白具有免疫活性,表达产物主要以包涵体的形式存在,进一步采用纯化试剂盒纯化P1蛋白做为诊断抗原.
將口蹄疫病毒(FMDV)結構蛋白基因P1的完整cDNA序列插入原覈錶達性載體pET-28α(+)中,穫得融閤錶達質粒pET-P1,轉化E.coli.BL21(DE3),經IPTG誘導,SDS-PAGE結果錶明,pET-P1穫得融閤錶達, Western Blot 檢測證實錶達的融閤蛋白具有免疫活性,錶達產物主要以包涵體的形式存在,進一步採用純化試劑盒純化P1蛋白做為診斷抗原.
장구제역병독(FMDV)결구단백기인P1적완정cDNA서렬삽입원핵표체성재체pET-28α(+)중,획득융합표체질립pET-P1,전화E.coli.BL21(DE3),경IPTG유도,SDS-PAGE결과표명,pET-P1획득융합표체, Western Blot 검측증실표체적융합단백구유면역활성,표체산물주요이포함체적형식존재,진일보채용순화시제합순화P1단백주위진단항원.
The complete gene encoding the structural protein of FMDV( P1 ) was subcloned into expression vector pET-28o( + ),resulting in the fusion expression plasmid pET-Pl .After transformed into E. coli (DE3) and induced by IPTG,the results of SDS-PAGE showed that the fusion protein was expressed in high level.The molecular weight of the fusion protein was 85 kDa and the expressed products were significant at inclusion body. Western blotting was performed to confirm that the expressed fusion protein could specifically react with antiserum against FMDV. The fusion protein were further purified and vaccined antigen based on the purified protein was developed.