生物技术通报
生物技術通報
생물기술통보
BIOTECHNOLOGY BULLETIN
2009年
12期
188-192
,共5页
纤维素酶%有机复合物H%滤纸%CMC
纖維素酶%有機複閤物H%濾紙%CMC
섬유소매%유궤복합물H%려지%CMC
Cellulase Composite%Organic Compounds H%Filter paper%CMC
采用3,5-二硝基水杨酸(DNS)为显色剂,滤纸和CMC为底物,测定纤维素酶的酶活.考察了在不同温度、pH值、反应时间、底物浓度等反应条件下,有机复合物H对纤维素酶滤纸酶活(FPA)和CMC酶活(CMCA)的影响.结果表明,有机复合物H对纤维素酶的FPA活性和CMCA活性均有一定的促进作用,且在不同条件下的促进效果有较大差异.
採用3,5-二硝基水楊痠(DNS)為顯色劑,濾紙和CMC為底物,測定纖維素酶的酶活.攷察瞭在不同溫度、pH值、反應時間、底物濃度等反應條件下,有機複閤物H對纖維素酶濾紙酶活(FPA)和CMC酶活(CMCA)的影響.結果錶明,有機複閤物H對纖維素酶的FPA活性和CMCA活性均有一定的促進作用,且在不同條件下的促進效果有較大差異.
채용3,5-이초기수양산(DNS)위현색제,려지화CMC위저물,측정섬유소매적매활.고찰료재불동온도、pH치、반응시간、저물농도등반응조건하,유궤복합물H대섬유소매려지매활(FPA)화CMC매활(CMCA)적영향.결과표명,유궤복합물H대섬유소매적FPA활성화CMCA활성균유일정적촉진작용,차재불동조건하적촉진효과유교대차이.
The test of the activity of cellulase was measured with 3,5-dinitrosalicylic acid (DNS) as reagent, filter paper and CMC as the substrate.The effect of composite organic compounds H to FPA and CMC cellulose activity was investigated in different temperature, pH, reaction time and the concentration of the substrate material.The results indicated that composite organic compounds H can distinctly promote the activity of CMC and FPA, and the difference varied according to the conditions.
