中华航海医学与高气压医学杂志
中華航海醫學與高氣壓醫學雜誌
중화항해의학여고기압의학잡지
CHINESE JOURNAL OF NAUTICAL MEDICINE AND HYPERBARIC MEDICINE
2011年
2期
105-108
,共4页
江叔奇%刘玉明%钱甜甜%何颖%蒋定文%沈先荣%葛卫红
江叔奇%劉玉明%錢甜甜%何穎%蔣定文%瀋先榮%葛衛紅
강숙기%류옥명%전첨첨%하영%장정문%침선영%갈위홍
鲨鱼%硫酸软骨素%咔唑法%Elson-Morgan法
鯊魚%硫痠軟骨素%咔唑法%Elson-Morgan法
사어%류산연골소%잡서법%Elson-Morgan법
Shark%Chondroitin sulfate%Carbazole method%Elson-Morgan method
目的 比较2种鲨鱼硫酸软骨素含量的测定方法.方法 以硫酸软骨素、氨基己糖为标准品,采用咔唑法、Elson-Morgan法分别测定鲨鱼软骨提取物中硫酸软骨素的含量.结果 咔唑法在4.710～47.100 μg/ml范围内,线性关系良好(r=0.9999),溶液在3 h内稳定性良好,相对标准差RSD=1.21%,重现性试验RSD=1.36%(n=6),精密度试验RSD=0.46%(n=6),平均回收率为99.60%,RSD=1.39%(n=6).Elson-Moran法在2.552～25.520μg/ml范围内,线性关系良好(r=0.9996),溶液在3 h内稳定性良好,RSD=1.62%,重现性试验RSD=2.61%(n=6),精密度试验RSD=1.07%(n=6),平均回收率为96.55%,RSD=2.43%(n=6).结论 咔唑法测定鲨鱼硫酸软骨素含量较Elson-Morgan 法简便、快速、准确,可作为测定鲨鱼硫酸软骨素含量的方法.
目的 比較2種鯊魚硫痠軟骨素含量的測定方法.方法 以硫痠軟骨素、氨基己糖為標準品,採用咔唑法、Elson-Morgan法分彆測定鯊魚軟骨提取物中硫痠軟骨素的含量.結果 咔唑法在4.710～47.100 μg/ml範圍內,線性關繫良好(r=0.9999),溶液在3 h內穩定性良好,相對標準差RSD=1.21%,重現性試驗RSD=1.36%(n=6),精密度試驗RSD=0.46%(n=6),平均迴收率為99.60%,RSD=1.39%(n=6).Elson-Moran法在2.552～25.520μg/ml範圍內,線性關繫良好(r=0.9996),溶液在3 h內穩定性良好,RSD=1.62%,重現性試驗RSD=2.61%(n=6),精密度試驗RSD=1.07%(n=6),平均迴收率為96.55%,RSD=2.43%(n=6).結論 咔唑法測定鯊魚硫痠軟骨素含量較Elson-Morgan 法簡便、快速、準確,可作為測定鯊魚硫痠軟骨素含量的方法.
목적 비교2충사어류산연골소함량적측정방법.방법 이류산연골소、안기기당위표준품,채용잡서법、Elson-Morgan법분별측정사어연골제취물중류산연골소적함량.결과 잡서법재4.710～47.100 μg/ml범위내,선성관계량호(r=0.9999),용액재3 h내은정성량호,상대표준차RSD=1.21%,중현성시험RSD=1.36%(n=6),정밀도시험RSD=0.46%(n=6),평균회수솔위99.60%,RSD=1.39%(n=6).Elson-Moran법재2.552～25.520μg/ml범위내,선성관계량호(r=0.9996),용액재3 h내은정성량호,RSD=1.62%,중현성시험RSD=2.61%(n=6),정밀도시험RSD=1.07%(n=6),평균회수솔위96.55%,RSD=2.43%(n=6).결론 잡서법측정사어류산연골소함량교Elson-Morgan 법간편、쾌속、준학,가작위측정사어류산연골소함량적방법.
Objective To compare the two methods for the determination of the shark chondroitin sulfate. Methods Using chondroitin sulfate and aminohexose as standards to determine the content of the shark chondroitin sulfate by the carbazole method and the Elson-Morgan method. Results Experiments showed that by the carbazole method, the concentration of the shark chondroitin sulfate was within the ranges of 4. 710-47. 100 μg/ml, displaying good linear relationship (r =0.9999) and good stability within 3 hours, with RSD = 1.21%, reproductive test RSD = 1. 36%( n=6), precision test RSD = 0. 46% (n = 6), and a recovery rate of 99.60% , RSD = 1. 39% ( n = 6). For the Elson-Morgan method, the concentration of the shark chondroitin sulfate was within the ranges of 2.552-25. 520 μg/ml, also displaying good linear relationship (r = 0. 9996) and good stability within 3 hours, with RSD = 1. 62% , reproductive test RSD = 2. 61% ( n = 6), precision test RSD = 1.07%(n = 6), and a recovery rate of 96. 55% ,RSD = 2.43% (n = 6). Conclusions When compared with the Elson-Morgan, the carbazole method was more simple, rapid and accurate and could be used a standard method for the determination of the content of shark chondroitin sulfate.
