CAJ | 학술논문

采用透射电子显微镜观察了虾夷盘扇贝(Patinopecten yessoensis)鳃组织细胞的超微结构,发现镉盐能胁迫鳃组织中的腮丝、细胞核和线粒体产生病变.利用双向凝胶电泳(2D-PAGE)优化分离扇贝鳃组织的全蛋白,获得约800个蛋白质斑点,并筛选出37个由于镉盐胁迫而产生的差异蛋白质斑点.选用基质辅助激光解吸离子化-飞行时间质谱(MALDI-TOF MS)技术和数据库检索鉴定差异蛋白,结果发现7个与镉毒性密切相关的蛋白质,即热休克蛋白70和β-淀粉酶等上调蛋白质及原肌球蛋白、肌动蛋白和钙活化核苷酸酶1等下调蛋白质.此外,还发现转录调节子Crp/Fnr家族为低表达蛋白质,而ABC转运子为高表达蛋白质.在这些差异蛋白中,部分蛋白质适合作为连续监测流动海水中镉污染程度及评价其危害性的蛋白指示物.
채용투사전자현미경관찰료하이반선패(Patinopecten yessoensis)새조직세포적초미결구,발현력염능협박새조직중적시사、 세포핵화선립체산생병변.이용쌍향응효전영(2D-PAGE)우화분리선패새조직적전단백,획득약800개단백질반점,병사선출37개유우력염협박이산생적차이단백질반점.선용기질보조격광해흡리자화-비행시간질보(MALDI-TOF MS)기술화수거고검색감정차이단백,결과발현7개여력독성밀절상관적단백질,즉열휴극단백70화β-정분매등상조단백질급원기구단백、기동단백화개활화핵감산매1등하조단백질.차외,환발현전록조절자Crp/Fnr가족위저표체단백질,이ABC전운자위고표체단백질.재저사차이단백중,부분단백질괄합작위련속감측류동해수중력오염정도급평개기위해성적단백지시물.
The organelle ultrastructure in gill tissue was observed by transmission electron microscope(TEM) in Patinopecten yessoensis (PY), finding the pathological changes of gill filaments, nucleus and mitochondria in the tissue exposed to CdCl_2(10 mg/L). The proteome of gill tissue in PY was perfectly separated by two-dimensional polyacrylamide gel electrophoresis(2D-PAGE), obtaining approximately 800 protein spots, and selecting 37 differential spots intimidated with cadmium salt in the gel. In addition, these differential proteins were identified by both matrix-assisted laser desorption/ionization-time of flight mass spectrometry(MALDI-TOF MS) and database searching. The results showed that 7 differential proteins tightly connected with the cadmium toxicity were considered to be up-regulated proteins such as heat shock protein 70 and β-amylase, and down-regulated proteins such as tropomyosin, actin and calcium activated nucleotidase 1. Moreover, transcriptional regulator Crp/Fnr family showed low expression, while ABC transporter showed high expression. We suggest that these differential proteins in part have strong potentials to be utilized as protein biomarkers for monitoring the pollution level of cadmium continuously and evaluating its risk to organisms.