水产科学
水產科學
수산과학
FISHERIES SCIENCE
2010年
3期
147-151
,共5页
宋晶%李晓东%吴垠%刘胥
宋晶%李曉東%吳垠%劉胥
송정%리효동%오은%류서
海蜇%耗氧率%排氨率%光照%温度%规格%窒息点
海蜇%耗氧率%排氨率%光照%溫度%規格%窒息點
해철%모양솔%배안솔%광조%온도%규격%질식점
jellyfish(Rhopilema esculenta)%OCR(oxygen consumption rate)%AER(ammonia excretion rate)%illumination%temperature%size%asphyxia point
试验在密闭(盐度25.1,pH 7.9~8.1)条件下进行,溶氧量和氨氮量分别采用Winkler法和纳氏试剂法测定.分析不同规格海蜇耗氧率和排氨率时,得出体质量(m)与伞径(D)的关系式为:m =0.0003 D~2-0.0007 D+0.0427,r~2=0.9913.幼蜇伞径从7 mm到35 mm,其耗氧率(OCR)和排氨率(AER)均随着伞径的增大而不断降低,分别经回归分析得到曲线:OCR=0.0063 D~2-0.3531 D+6.1533,r~2=0.9751和AER=0.4221 D~2-26.585 D+518.83,r~2=0.9587.光照影响试验结果表明:各组别光照下的耗氧率、排氨率均显著大于黑暗条件(P<0.01).分析结果表明,只有20 ℃和25 ℃耗氧率差异不显著(P>0.05),而其余各温度间的耗氧率差异均显著(P<0.05);15 ℃与25 ℃,20 ℃与25 ℃之间排氨率差异不显著(P>0.05),而升温至30 ℃时,幼蜇代谢明显加快,尤其是排氨率上升较快,与其余各温度间的排氨率差异均极显著(P<0.01).对幼蜇在光照和黑暗条件下的窒息点进行了测定,发现溶氧小于0.45 mg/L时,试验所采用的伞径为15 mm的幼蜇半数窒息死亡.
試驗在密閉(鹽度25.1,pH 7.9~8.1)條件下進行,溶氧量和氨氮量分彆採用Winkler法和納氏試劑法測定.分析不同規格海蜇耗氧率和排氨率時,得齣體質量(m)與傘徑(D)的關繫式為:m =0.0003 D~2-0.0007 D+0.0427,r~2=0.9913.幼蜇傘徑從7 mm到35 mm,其耗氧率(OCR)和排氨率(AER)均隨著傘徑的增大而不斷降低,分彆經迴歸分析得到麯線:OCR=0.0063 D~2-0.3531 D+6.1533,r~2=0.9751和AER=0.4221 D~2-26.585 D+518.83,r~2=0.9587.光照影響試驗結果錶明:各組彆光照下的耗氧率、排氨率均顯著大于黑暗條件(P<0.01).分析結果錶明,隻有20 ℃和25 ℃耗氧率差異不顯著(P>0.05),而其餘各溫度間的耗氧率差異均顯著(P<0.05);15 ℃與25 ℃,20 ℃與25 ℃之間排氨率差異不顯著(P>0.05),而升溫至30 ℃時,幼蜇代謝明顯加快,尤其是排氨率上升較快,與其餘各溫度間的排氨率差異均極顯著(P<0.01).對幼蜇在光照和黑暗條件下的窒息點進行瞭測定,髮現溶氧小于0.45 mg/L時,試驗所採用的傘徑為15 mm的幼蜇半數窒息死亡.
시험재밀폐(염도25.1,pH 7.9~8.1)조건하진행,용양량화안담량분별채용Winkler법화납씨시제법측정.분석불동규격해철모양솔화배안솔시,득출체질량(m)여산경(D)적관계식위:m =0.0003 D~2-0.0007 D+0.0427,r~2=0.9913.유철산경종7 mm도35 mm,기모양솔(OCR)화배안솔(AER)균수착산경적증대이불단강저,분별경회귀분석득도곡선:OCR=0.0063 D~2-0.3531 D+6.1533,r~2=0.9751화AER=0.4221 D~2-26.585 D+518.83,r~2=0.9587.광조영향시험결과표명:각조별광조하적모양솔、배안솔균현저대우흑암조건(P<0.01).분석결과표명,지유20 ℃화25 ℃모양솔차이불현저(P>0.05),이기여각온도간적모양솔차이균현저(P<0.05);15 ℃여25 ℃,20 ℃여25 ℃지간배안솔차이불현저(P>0.05),이승온지30 ℃시,유철대사명현가쾌,우기시배안솔상승교쾌,여기여각온도간적배안솔차이균겁현저(P<0.01).대유철재광조화흑암조건하적질식점진행료측정,발현용양소우0.45 mg/L시,시험소채용적산경위15 mm적유철반수질식사망.
Oxygen consumption and ammonia excretion were determined by the Winkler method and the Nessler's reagent method in juvenile jellyfish Rhopilema esculenta with various sizes under different ambient illumination, pH 7.9~8.1 and salinity of 25.1. The relationship between dry weight(m)and the bell diameter(D)was found to be represented as the equation:m=0.0003 D~2-0.0007 D+0.0427, r~2=0.9913. There was a negative regression function among oxygen consumption rate(OCR), ammonia excretion rate(AER)and the bell diameter(D):OCR=0.0063 D~2-0.3531 D+6.1533, r~2=0.9751; AER=0.4221 D~2-26.585 D+518.83, r~2= 0.9587. The juvenile jellyfish had significantly greater OCR and AER under light than under darkness(P<0.01). The OCR and AER showed a tendency to increase with the increase in water temperature. The significant increases in AER was only found in 30 ℃ group and other three groups(P<0.01)and the significant increases in OCR were observed in the high water temperature groups except from the 20 ℃ group and the 25 ℃ group(P<0.05). The asphyxia was found at the dissolved oxygen concentration of 0.45 mg/L for the juvenile jellyfish with bell diameter of 15 mm under light than under darkness.