中国地方病学杂志
中國地方病學雜誌
중국지방병학잡지
CHINESE JOURNAL OF ENDEMIOLOGY
2010年
5期
485-486
,共2页
朱参胜%于曰祥%王欢%刘雯
硃參勝%于曰祥%王歡%劉雯
주삼성%우왈상%왕환%류문
大骨节病%关节损伤%白细胞介素-2%干扰素-γ
大骨節病%關節損傷%白細胞介素-2%榦擾素-γ
대골절병%관절손상%백세포개소-2%간우소-γ
Kashin-Beck disease%Joint injure%Interleukin-2%Interferon-γ
目的 观察白细胞介素-2(IL-2)和干扰素-γ(IFN-γ)在大骨节病(KBD)关节损伤中的变化,探讨IL-2和IFN-r在KBD关节损伤中的作用.方法 按照<大骨节病诊断标准>(GB 16003-1995),48例KBD患者和26例健康人(对照组)均来自陕西陇县KBD病区.KBD组男、女各24例,年龄在40～65岁,平均年龄51岁,采集静脉血48份和关节滑液28份(男、女各14份).对照组男、女各13人,年龄40～65岁,平均年龄49岁,采集其静脉血26份.血清、关节滑液中IL-2和IFN-γ采用酶联免疫吸附法(ELISA)测定.结果 对照组和KBD患者血清中IL-2中位数分别为46.8 ng/L和55.7 ng/L,IFN-γ分别为52.3 ng/L和48.8 ng/L,二者比较差异无统计学意义(t值分别为0.62、0.70,P均＞0.05).KBD患者关节滑液中IL-2中位数为48.3 ng/L,IFN-γ为44.1g/L,关节滑液与血清比较差异无统计学意义(t值分别为0.69、1.72,P均＞0.05).结论 KBD关节损伤患者血清和关节液中IL-2和IFN-γ均无显著性升高,表明在KBD的关节损伤过程中,IL-2和IFN-γ均未参与.
目的 觀察白細胞介素-2(IL-2)和榦擾素-γ(IFN-γ)在大骨節病(KBD)關節損傷中的變化,探討IL-2和IFN-r在KBD關節損傷中的作用.方法 按照<大骨節病診斷標準>(GB 16003-1995),48例KBD患者和26例健康人(對照組)均來自陝西隴縣KBD病區.KBD組男、女各24例,年齡在40～65歲,平均年齡51歲,採集靜脈血48份和關節滑液28份(男、女各14份).對照組男、女各13人,年齡40～65歲,平均年齡49歲,採集其靜脈血26份.血清、關節滑液中IL-2和IFN-γ採用酶聯免疫吸附法(ELISA)測定.結果 對照組和KBD患者血清中IL-2中位數分彆為46.8 ng/L和55.7 ng/L,IFN-γ分彆為52.3 ng/L和48.8 ng/L,二者比較差異無統計學意義(t值分彆為0.62、0.70,P均＞0.05).KBD患者關節滑液中IL-2中位數為48.3 ng/L,IFN-γ為44.1g/L,關節滑液與血清比較差異無統計學意義(t值分彆為0.69、1.72,P均＞0.05).結論 KBD關節損傷患者血清和關節液中IL-2和IFN-γ均無顯著性升高,錶明在KBD的關節損傷過程中,IL-2和IFN-γ均未參與.
목적 관찰백세포개소-2(IL-2)화간우소-γ(IFN-γ)재대골절병(KBD)관절손상중적변화,탐토IL-2화IFN-r재KBD관절손상중적작용.방법 안조<대골절병진단표준>(GB 16003-1995),48례KBD환자화26례건강인(대조조)균래자협서롱현KBD병구.KBD조남、녀각24례,년령재40～65세,평균년령51세,채집정맥혈48빈화관절활액28빈(남、녀각14빈).대조조남、녀각13인,년령40～65세,평균년령49세,채집기정맥혈26빈.혈청、관절활액중IL-2화IFN-γ채용매련면역흡부법(ELISA)측정.결과 대조조화KBD환자혈청중IL-2중위수분별위46.8 ng/L화55.7 ng/L,IFN-γ분별위52.3 ng/L화48.8 ng/L,이자비교차이무통계학의의(t치분별위0.62、0.70,P균＞0.05).KBD환자관절활액중IL-2중위수위48.3 ng/L,IFN-γ위44.1g/L,관절활액여혈청비교차이무통계학의의(t치분별위0.69、1.72,P균＞0.05).결론 KBD관절손상환자혈청화관절액중IL-2화IFN-γ균무현저성승고,표명재KBD적관절손상과정중,IL-2화IFN-γ균미삼여.
Objective To investigate the changes of interleukin(IL)-2 and interferon(IFN)-γin serum and synovial fluid of patients with joint injure with Kashin-Beck disease(KBD), and study the roles of IL-2 and IFN-γ in KBD joint injure. Methods In accordance with the "Diagnostic Criteria of Kashin-Beck disease"(GB16003-1995),48 cases of KBD patients and 26 healthy people(control group) from KBD endemic area in Long county Shaanxi province were enrolled in the study. KBD patient were 24 males and 24 females, respectively, aged 40 to 65 years (mean age 51 years). Forty-eight serum specimens and 28 synovial fluid specimens of patients(14 males and 14 females,respectively) were collected. Healthy control group were 13 males and 13 females, respectively. Twenty-six serum specimens of healthy controls were collected. Serum and synovial fluid IL-2 and IFN-γ levels were determined by enzyme-linked immunosorbent assay(ELISA). Results In healthy controls and KBD patients, the midian of serum IL-2 were 46.8 ng/L and 55.7 ng/L, respectively, and IFN-γ were 52.3 ng/L and 48.8 ng/L, respectively. The difference was not statistically significant between healthy controls and KBD patients(t = 0.62, 0.70, all P ＞ 0.05).In synovial fluid of KBD patient, the midian of IL-2 and IFN-γwere 48.3 ng/L and 44.1 ng/L, respectively. The difference was not statistically significant between serum and synovial fluid in KBD patients(t = 0.69, 1.72, all P ＞0.05). Conclusion Serum and synovial fluid IL-2 and IFN-γare not significantly increased in KBD patients with articular damage, indicating that IL-2 and IFN-γare not involved in KBD joint injury.
