CAJ | 학술논문

目的观察中国恒河猴感染猴免疫缺陷病毒(simian immunodeficiency virus,SIV)后病毒学和免疫学基本指标的变化规律,探讨中国恒河猴用于艾滋病模型时外周血白细胞(WBC)和CD4+T细胞比例的标准.方法以SIVmac251感染36只中国恒河猴,于感染前1天及感染后1～8周每周及感染后第10周分别采集外周静脉血,检测血常规、外周血淋巴细胞亚群和病毒载量.结果除WBC计数变化不显著外,其余检测指标均在感染后第1、2周时变化最为剧烈；WBC计数在(4～10)×106个/ml之间结合外周血CD4'T细胞比例高于25％的可作为艾滋病模型的选猴标准.结论为应用中国恒河猴进行艾滋病研究提供了有益的信息.
목적 관찰중국항하후감염후면역결함병독(simian immunodeficiency virus,SIV)후병독학화면역학기본지표적변화규률,탐토중국항하후용우애자병모형시외주혈백세포(WBC)화CD4+T세포비례적표준.방법 이SIVmac251감염36지중국항하후,우감염전1천급감염후1～8주매주급감염후제10주분별채집외주정맥혈,검측혈상규、외주혈림파세포아군화병독재량.결과 제WBC계수변화불현저외,기여검측지표균재감염후제1、2주시변화최위극렬；WBC계수재(4～10)×106개/ml지간결합외주혈CD4'T세포비례고우25％적가작위애자병모형적선후표준.결론 위응용중국항하후진행애자병연구제공료유익적신식.
Objective To observe the virological and immunological items in peripheral blood of Chinese-origin Rhesus macaques infected with SIVmac251.The normal levels of WBC and CD4+T cell ratio for healthy Chinese-origin Rhesus macaques that suitable for simian AIDS modeling also investigated.Methods Thirty-six Chinese-origin Rhesus macaques were intravenously infected with SIVmac251.Blood samples were collected at 10 time points respectively include 1 day before SIV infection,every week within 1-8th week and then in tenth week post infection.Blood routine,peripheral blood T lymphocyte subsets,plasma viral load were tested.Results The most significant changes of the tested items were appeared in 1 or 2 weeks post SIV infection,while the WBC counts didn't show marked changes in all the time points tested.WBC counts ranged from 4×106/ml to 10×106/ml and the CD4+T cells ratio high than 25％ are the suitable levels for simian AIDS modeling.Conclusion This research provides necessary and beneficial informations to the usage of Chinese-origin Rhesus macaques in AIDS research.