中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2012年
24期
1715-1718
,共4页
陈浩%梅运清%Michael A Solomon
陳浩%梅運清%Michael A Solomon
진호%매운청%Michael A Solomon
心脏移植%模型,动物%大鼠
心髒移植%模型,動物%大鼠
심장이식%모형,동물%대서
Heart transplantation%Models,animal%Rats
目的 总结在美国国立卫生研究院临床中心建立大鼠腹腔内心脏移植模型的经验,探讨手术成功的主要影响因素.方法 以80只Dark Agouti大鼠为供体,70只Brown Norway大鼠为受体,制作大鼠心脏移植模型.以连续20例手术死亡率低于15％且无明显合并症为手术合格标准.结果 为达到合格标准,共施行了10例大鼠供体手术,70例大鼠移植手术.最后20例移植手术成功率为95％,平均手术时间为55 min,供心平均缺血时间为35 min.结论 精准的显微外科技术,加上标准化手术训练和严格的动物管理,是保证手术成功率的关键因素.
目的 總結在美國國立衛生研究院臨床中心建立大鼠腹腔內心髒移植模型的經驗,探討手術成功的主要影響因素.方法 以80隻Dark Agouti大鼠為供體,70隻Brown Norway大鼠為受體,製作大鼠心髒移植模型.以連續20例手術死亡率低于15％且無明顯閤併癥為手術閤格標準.結果 為達到閤格標準,共施行瞭10例大鼠供體手術,70例大鼠移植手術.最後20例移植手術成功率為95％,平均手術時間為55 min,供心平均缺血時間為35 min.結論 精準的顯微外科技術,加上標準化手術訓練和嚴格的動物管理,是保證手術成功率的關鍵因素.
목적 총결재미국국립위생연구원림상중심건립대서복강내심장이식모형적경험,탐토수술성공적주요영향인소.방법 이80지Dark Agouti대서위공체,70지Brown Norway대서위수체,제작대서심장이식모형.이련속20례수술사망솔저우15％차무명현합병증위수술합격표준.결과 위체도합격표준,공시행료10례대서공체수술,70례대서이식수술.최후20례이식수술성공솔위95％,평균수술시간위55 min,공심평균결혈시간위35 min.결론 정준적현미외과기술,가상표준화수술훈련화엄격적동물관리,시보증수술성공솔적관건인소.
Objective To review the experiences and key factors of establishing an abdominal cardiac transplantation model in rats at National Institutes of Health Clinical Center.Methods Dark Agouti rats served as donor while Brown Norway rats as recipient.Cardiac transplantations were performed with a modified version of heterotopic cardiac transplantation.Proficiency was defined as 20 consecutive operations with ＜ 15％ mortality and no significant major co-morbidities.Results We performed 10 donor harvestalone operations and 70 transplantations to reach the proficiency level The survival rate of the last 20 consecutive surgeries was 95％.The mean total operative duration was 55 ruin and mean ischemic time of donor hearts 35 min.Conclusion To ensure a consistently high operative success rate,a surgeon must not only be proficient in micro-surgical skills,but also closely follow the standardized training procedures and strictly observe the animal care and use guidelines.
