中华创伤骨科杂志
中華創傷骨科雜誌
중화창상골과잡지
CHINESE JOURNAL OF ORTHOPAEDIC TRAUMA
2011年
6期
565-570
,共6页
许道荣%金丹%肖庭辉%余斌
許道榮%金丹%肖庭輝%餘斌
허도영%금단%초정휘%여빈
软骨细胞%转化生长因子%机械拉伸形变%凋亡细胞因子%兔
軟骨細胞%轉化生長因子%機械拉伸形變%凋亡細胞因子%兔
연골세포%전화생장인자%궤계랍신형변%조망세포인자%토
Chondrocytes%Transforming growth factors%Mechanical stress%Apoptotic gene expression%Rabbits
目的 探讨兔软骨细胞受到拉伸机械形变后金属蛋白酶(MMP)及转化生长因子-β1(TGF-β1)的影响.方法 取兔原代关节软骨细胞培养方法,利用FLEXERCELL-5000T细胞拉伸系统对单层培养细胞施加拉伸形变,其中分为对照组、低幅度(5%)拉伸组及高幅度(15%)拉伸组,每天作用8 h,拉伸5 d,分别取3、5 d时细胞行实时定量PCR(qRT-PCR)检测MMP3、MMP13、ADAMTS-5、TGF-β1的基因表达.结果 拉伸组MMP3、ADAMTS-5基因水平3 d时拉伸形变导致MMP3、ADAMTS-5均显著升高(P=0.002),5 d时各组差异有统计学意义.3 d时各拉伸组中MMP13均高于对照组,5 d时高幅度拉伸组高于低幅度拉伸组.5 d时拉伸组中TGF-β1均高于对照组,低幅度拉伸组高于高幅度拉伸组.结论 本实验结果明确了软骨细胞中凋亡细胞因子及TGF的基因水平受不同拉伸幅度而旱现不同趋势.TGF-β1在软骨细胞拉伸形变的修复中起到重要作用.
目的 探討兔軟骨細胞受到拉伸機械形變後金屬蛋白酶(MMP)及轉化生長因子-β1(TGF-β1)的影響.方法 取兔原代關節軟骨細胞培養方法,利用FLEXERCELL-5000T細胞拉伸繫統對單層培養細胞施加拉伸形變,其中分為對照組、低幅度(5%)拉伸組及高幅度(15%)拉伸組,每天作用8 h,拉伸5 d,分彆取3、5 d時細胞行實時定量PCR(qRT-PCR)檢測MMP3、MMP13、ADAMTS-5、TGF-β1的基因錶達.結果 拉伸組MMP3、ADAMTS-5基因水平3 d時拉伸形變導緻MMP3、ADAMTS-5均顯著升高(P=0.002),5 d時各組差異有統計學意義.3 d時各拉伸組中MMP13均高于對照組,5 d時高幅度拉伸組高于低幅度拉伸組.5 d時拉伸組中TGF-β1均高于對照組,低幅度拉伸組高于高幅度拉伸組.結論 本實驗結果明確瞭軟骨細胞中凋亡細胞因子及TGF的基因水平受不同拉伸幅度而旱現不同趨勢.TGF-β1在軟骨細胞拉伸形變的脩複中起到重要作用.
목적 탐토토연골세포수도랍신궤계형변후금속단백매(MMP)급전화생장인자-β1(TGF-β1)적영향.방법 취토원대관절연골세포배양방법,이용FLEXERCELL-5000T세포랍신계통대단층배양세포시가랍신형변,기중분위대조조、저폭도(5%)랍신조급고폭도(15%)랍신조,매천작용8 h,랍신5 d,분별취3、5 d시세포행실시정량PCR(qRT-PCR)검측MMP3、MMP13、ADAMTS-5、TGF-β1적기인표체.결과 랍신조MMP3、ADAMTS-5기인수평3 d시랍신형변도치MMP3、ADAMTS-5균현저승고(P=0.002),5 d시각조차이유통계학의의.3 d시각랍신조중MMP13균고우대조조,5 d시고폭도랍신조고우저폭도랍신조.5 d시랍신조중TGF-β1균고우대조조,저폭도랍신조고우고폭도랍신조.결론 본실험결과명학료연골세포중조망세포인자급TGF적기인수평수불동랍신폭도이한현불동추세.TGF-β1재연골세포랍신형변적수복중기도중요작용.
Objective To explore the effects of cyclic tensile stresses of different magnitudes on expressions of apoptosis cytokines and transforming growth factors (TGF) in chondrocytes. Methods Classical methods were used to culture primary chondrocytes in New Zealand rabbits. Mechanical stresses were exerted onto the mono-layer cells cultured by the FLEXERCELL-5000T system. The experiment was conducted in 3 groups: low magnitude stress group (5% ), high magnitude stress group (15% ) and control group. The stresses were applied for 5 days and 8 hours per day. The cultured cells were harvested on days 3 and S to measure gene copies of MMP3 (matrix metalloproteinases), MMP13, ADMATS-5 (a disintegrin and metalloproteinase with thrombospondin motifs), and TGF-β1 by quantitative RT-PCR. Results Expressions of ADMATS-5 and MMP3 were significantly enhanced in each stress group on day 3 but weakened on day 5 ( P = 0. 002). Expressions of MMP13 were significantly higher in the stress groups than in the control group on day 3, and significantly higher in the high magnitude stress group than in the low one on day 5 ( P = 0. 002) .Levels of TGF-β1 on day 5 were significantly higher in the stress groups than in the control group, and higher in the low magnitude stress group than in the high one (P = 0. 002). Conclusions Gene expressions of apoptosis cytokines and TGF in chondrocytes are differently influenced by cyclic tensile stresses of different magnitudes. TGF -β1 may play an important role in pathological repair of the deformed chondrocytes following tensile stress.
