CAJ | 학술논문

目的研究溴氰菊酯(DM)亚慢性染毒对小鼠神经行为和海马谷氨酸受体(NMDA)表达的影响.方法清洁级雌性昆明小鼠60只,分为4组,通过灌胃连续染毒60 d,热板试验、转棒试验、抓力试验和后肢支撑力测试检测小鼠感觉、运动行为的改变；自主活动能力测试反应小鼠中枢神经系统的功能状态；避暗试验检测小鼠学习、记忆行为的改变；RQ-PCR技术检测海马NMDA受体mRNA的表达.结果 DM亚慢性染毒后小鼠在感觉、运动功能方面未出现明显改变(P＞0.05)；自主活动能力测试染毒剂量组小鼠平均自主活动次数为(93±18)次、(107±13)次、(105±22)次,与溶剂对照组比较,中、高剂量组小鼠平均自主活动次数增加,差异有统计学意义(P＜0.05).避暗试验染毒剂量组小鼠潜伏期分别为(175.4±38.4)s、(146.4±51.2)s、(132.3 ±45.0)s,失败次数分别为(0.7±0.3)次、(1.4±0.5)次、(1.8±0.9)次,与溶剂对照组比较,高剂量组小鼠潜伏期缩短,中、高剂量组失败次数增加,差异有统计学意义(P＜0.05)；与溶剂对照组比较,中、高剂量组小鼠海马NR1、NR2A mRNA相对表达水平均增高(P＜0.05),高剂量组NR2B mRNA的相对表达水平降低(P＜0.05).结论 DM亚慢性染毒可引起小鼠自主活动次数增加、学习和记忆功能损伤,并影响海马神经元NMDA受体mRNA的表达.
목적 연구추청국지(DM)아만성염독대소서신경행위화해마곡안산수체(NMDA)표체적영향.방법 청길급자성곤명소서60지,분위4조,통과관위련속염독60 d,열판시험、전봉시험、조력시험화후지지탱력측시검측소서감각、운동행위적개변；자주활동능력측시반응소서중추신경계통적공능상태；피암시험검측소서학습、기억행위적개변；RQ-PCR기술검측해마NMDA수체mRNA적표체.결과 DM아만성염독후소서재감각、운동공능방면미출현명현개변(P＞0.05)；자주활동능력측시염독제량조소서평균자주활동차수위(93±18)차、(107±13)차、(105±22)차,여용제대조조비교,중、고제량조소서평균자주활동차수증가,차이유통계학의의(P＜0.05).피암시험염독제량조소서잠복기분별위(175.4±38.4)s、(146.4±51.2)s、(132.3 ±45.0)s,실패차수분별위(0.7±0.3)차、(1.4±0.5)차、(1.8±0.9)차,여용제대조조비교,고제량조소서잠복기축단,중、고제량조실패차수증가,차이유통계학의의(P＜0.05)；여용제대조조비교,중、고제량조소서해마NR1、NR2A mRNA상대표체수평균증고(P＜0.05),고제량조NR2B mRNA적상대표체수평강저(P＜0.05).결론 DM아만성염독가인기소서자주활동차수증가、학습화기억공능손상,병영향해마신경원NMDA수체mRNA적표체.
Objective To study the effect of sub-chronic exposure to deltamethrin(DM) on neural behavior and expression of NMDA receptor in hippocampus of mice.Methods 60 Female SPF Kunming mice were divided into 4 groups and given DM 60 days by gavage.Hot-plate,rotarod,grip strength,hing limb landing foot splay were used to examine the sensory and motor change of mice.Autonomic activity test was used for detecting the functional status of the central nervous system in mice.Passive avoidance test for detection of the behavior changes of learning and memory,and RQ-PCR was employed to measure the expression of NMDA receptor in hippocampus of mice.Results The behavior of sensory and motor of mice sub-chronic exposure to deltamethrin did not have changes significantly(P ＞ 0.05 ).In the test of autonomic activity test,the average of autonomic activity times were (93 ± 18) times,(107 ± 13) times,(105 ±22) times.Compared with the control group,the average of autonomic activity times in middle-and high-dose groups were increased significantly (P ＜ 0.05 ).The latent periods in poisoning groups were (175.4 ±38.4) s,(146.4 ±51.2)s,(132.3 ±45.0) s,and the error times were (0.7 ±0.3)times,( 1.4 ± 0.5 ) times,( 1.8 ± 0.9) times.Compared with the control group,latent periods of high-dose group were decreased and the error times of middle-and high-dose groups were increased significantly (P ＜ 0.05 ).Compared with the control group,the relative expression levels of NR1 and NR2A mRNA in hippocampus of middle and high-dose groups were increased significantly (P＜ 0.05 ),and the relative expression level of NR2B mRNA in highdose group was decreased significantly(P ＜ 0.05).Conclusion Sub-chronic exposure to DM could increase the excitability of mice,damage the function of learning and memory,and influence the expression of NMDA receptor in hippocampus of mice.