CAJ | 학술논문

目的探讨胰岛素样生长因子2(IGF-2)外显子9 CpG岛甲基化状态与IGF-2基因表达的关系,以进一步探究肾母细胞瘤发生机制.方法应用甲基化敏感性限制性内切酶PCR和等位基因特异性IGF-2基因表达分析检测42例肾母细胞瘤及相应正常组织的IGF-2外显子9甲基化状态及IGF-2基因表达.结果肾母细胞瘤IGF-2外显子9甲基化率为15%,瘤旁组织为97%,两者差异有统计学意义(P<0.01),病理分型间差异亦有统计学意义(P<0.01).外显子9去甲基化组织中IGF-2双等位基因表达率显著高于甲基化的组织(P<0.05).结论肾母细胞瘤IGF-2外显子9去甲基化可能是引起IGF双等位基因表达,进而导致肿瘤发生的原因之一.
목적 탐토이도소양생장인자2(IGF-2)외현자9 CpG도갑기화상태여IGF-2기인표체적관계,이진일보탐구신모세포류발생궤제.방법 응용갑기화민감성한제성내절매PCR화등위기인특이성IGF-2기인표체분석검측42례신모세포류급상응정상조직적IGF-2외현자9갑기화상태급IGF-2기인표체.결과 신모세포류IGF-2외현자9갑기화솔위15%,류방조직위97%,량자차이유통계학의의(P<0.01),병리분형간차이역유통계학의의(P<0.01).외현자9거갑기화조직중IGF-2쌍등위기인표체솔현저고우갑기화적조직(P<0.05).결론 신모세포류IGF-2외현자9거갑기화가능시인기IGF쌍등위기인표체,진이도치종류발생적원인지일.
Object To investigate the correlation of IGF-2 exon 9 CpG island methylation and the expression of IGF-2,and further explore the mechanism of Wilms tumor. Methods The IGF-2 exon 9 methylation status and IGF2 gene expression in 42 cases of Wilms tumor and corresponding normal tissues were detected by using methylation-sensitive restriction enzyme PCR and allele-specific IGF2 gene expression analysis.Results IGF-2 exon 9 methylation rate was 15% in W ilms tumor group, and 97% in normal tissue group. The difference was significant (P < 0.01), and the difference in pathological type was also significantly different (P < 0.01). Exon 9 unmethylated tissues showing biallelic expressions of IGF-2 were significantly higher than that of methylated tissues (P < 0.05). Conclusion IGF-2 exon 9 unmethylation may be the cause of biallelic expression of IGF, which contritues to tumorigenesis in Wilms tumor.