CAJ | 학술논문

目的利用大鼠脑室内注射低剂量脂多糖(lipopolysaccharide,LPS)(10μg)诱导全脑炎症,长时间观察大鼠行为学、黑质部位小胶质细胞激活及多巴胺(Dopamine,DA)能神经元的变化,研究小胶质细胞激活与DA能神经元损害的关系,探讨炎症在帕金森病(Parkinson's disease,PD)发病机制中的作用.方法 50只雄性SD大鼠分为生理盐水对照组和10 μg LPS组.大鼠右侧脑室内注射生理盐水或10 μgLPS,术后不同时间观察大鼠行为学改变,免疫组化方法观察大鼠黑质部位小胶质细胞激活情况及酪氨酸羟化酶( tyrosine hydroxylase,TH)阳性神经元的变化.Fluoro-Jade B(FJB)染色检测大鼠黑质部位神经元变性情况.结果 ①注射后4周至36周两组大鼠平均运动速度相近.注射后40周时10μg LPS组大鼠平均运动速度比生理盐水对照组降低24.6％ (P＞0.05).②注射后24周和40周,10 μg LPS组大鼠黑质部位可见明显激活的OX-42阳性小胶质细胞,生理盐水对照组未见明显激活的OX-42阳性小胶质细胞.两组大鼠黑质部位均未发现OX-6阳性小胶质细胞.③注射后24周和40周,10 μg LPS组大鼠黑质部位TH阳性神经元数目比生理盐水对照组分别减少了24.2％ (t=4.803,P＜0.01)和27.6％ (t=3.212,P＜0.01).④两组大鼠黑质部均未见FJB阳性染色神经元.结论低剂量LPS脑室内注射可造成大鼠黑质部位小胶质细胞长期慢性激活及DA能神经元慢性损害.LPS诱导的全脑炎症在短时间内不会引起黑质DA能神经元变性坏死.脑室内注射10μg LPS模型能很好模拟DA能神经元慢性变性的特点,是研究PD的较好模型.
목적 이용대서뇌실내주사저제량지다당(lipopolysaccharide,LPS)(10μg)유도전뇌염증,장시간관찰대서행위학、흑질부위소효질세포격활급다파알(Dopamine,DA)능신경원적변화,연구소효질세포격활여DA능신경원손해적관계,탐토염증재파금삼병(Parkinson's disease,PD)발병궤제중적작용.방법 50지웅성SD대서분위생리염수대조조화10 μg LPS조.대서우측뇌실내주사생리염수혹10 μgLPS,술후불동시간관찰대서행위학개변,면역조화방법관찰대서흑질부위소효질세포격활정황급락안산간화매( tyrosine hydroxylase,TH)양성신경원적변화.Fluoro-Jade B(FJB)염색검측대서흑질부위신경원변성정황.결과 ①주사후4주지36주량조대서평균운동속도상근.주사후40주시10μg LPS조대서평균운동속도비생리염수대조조강저24.6％ (P＞0.05).②주사후24주화40주,10 μg LPS조대서흑질부위가견명현격활적OX-42양성소효질세포,생리염수대조조미견명현격활적OX-42양성소효질세포.량조대서흑질부위균미발현OX-6양성소효질세포.③주사후24주화40주,10 μg LPS조대서흑질부위TH양성신경원수목비생리염수대조조분별감소료24.2％ (t=4.803,P＜0.01)화27.6％ (t=3.212,P＜0.01).④량조대서흑질부균미견FJB양성염색신경원.결론 저제량LPS뇌실내주사가조성대서흑질부위소효질세포장기만성격활급DA능신경원만성손해.LPS유도적전뇌염증재단시간내불회인기흑질DA능신경원변성배사.뇌실내주사10μg LPS모형능흔호모의DA능신경원만성변성적특점,시연구PD적교호모형.
Objective To investigate the long-term effect of inflammation on behavior,microglias and dopaminergic (DA) neurons in the substantia nigra of intracephalic inflammation rat models induced by intracerebroventricular injection of low-dose(10μg) lipopolysaccharide (LPS).To analyze the relationship between activation of microglias and DA neurons degeneration in order to explore the mechanism of inflammation in the progressive process of Parkinson' s disease (PD).Methods 50 healthy male SD rats were randomly assigned into saline-injected control group and 10μg LPS-injected group.All injections were made intracerebroventricularly on right side of rats with saline or LPS.Moving speed was measured at different time points.At 24 weeks and 40 weeks after saline or LPS injection,specific antibodies of OX-42 and OX-6 were used separately to detect the changes of microglia in the substantia nigra of rat.The changes in morphology and numbers of substantia nigra DA neurons were observed by tyrosine hydroxylase(TH) immunohistochemical staining.The expression and distribution of the degenerated neurons in substantia nigra were detected by using Fluoro-Jade B(FJB).Results ①Analysis of moving speed sho wed that the moving speed of 10μg LPS-injected group rats and saline-injected group rats was similar from 4 weeks to 36 weeks after injection.At 40 weeks post injection,moving speed of 10μg LPS-injected group rats decreased by 24.6％ compared with that of saline-injected group rats (P＞ 0.05 ).②At 24 weeks and 40 weeks after injection,there were many activated OX-42 positive microglias in the substantia nigra of 10μg LPS-injected group rats,but there was almost no significant activated OX-42 positive microglia in saline-injected group.OX-6 positive microglias were not found in the substantia nigra of both of two groups.③At 24 weeks and 40 weeks post injection,the number of TH-positive neurons in the substantia nigra of 10μg LPS-injected group rats decreasedby 24.2％ ( t=4.803,P＜0.01) and 27.6％ ( t=3.212,P＜0.01) respectively compared with those of salineinjected group.④ There was no FJB positive neurons in the substantia nigra of the two group rats.Conclusion Intraventricular injection of low-dose LPS ( l0μg) in rats may induce long-term activation of microglias and chronic degeneration of DA neurons in the subs tantia nigra of rats although the necrosis are not occurs to DA neurons till 40 weeks post LPS injection.Intraventricular injection of low-dose LPS in rats could be ideal model to study the mechanism of chronic degeneration of DA neurons in PD.