CAJ | 학술논문

目的探讨人胃癌组织中hsa-miR-124a基因启动子区的DNA甲基化状态与Rb和CDK6蛋白表达及临床病理因素的关系.方法用甲基化特异性PCR(MSP)方法检测手术切除的30例胃癌及相应癌旁组织的hsa-miR-124a DNA甲基化状态;采用免疫组织化学染色研究Rb和CDK6蛋白在胃癌组织中的表达情况.结果胃癌组织中hsa-miR-124a总体甲基化率为73.3%(22/30),明显高于癌旁组织的10.0%(3/30)(P＜0.05),其甲基化状态与肿瘤大小、分化程度、淋巴结转移、侵犯程度等临床病理因素有关(P＜0.01).hsa-miR-124a甲基化与Rb蛋白和CDK6蛋白表达之间呈正相关(P＜0.05).结论胃癌组织中hsa-miR-124a处于高甲基化状态,这种高甲基化可能通过增强Rb及CDK6蛋白的表达来促进胃癌的恶性增殖.
목적 탐토인위암조직중hsa-miR-124a기인계동자구적DNA갑기화상태여Rb화CDK6단백표체급림상병리인소적관계.방법 용갑기화특이성PCR(MSP)방법검측수술절제적30례위암급상응암방조직적hsa-miR-124a DNA갑기화상태;채용면역조직화학염색연구Rb화CDK6단백재위암조직중적표체정황.결과위암조직중hsa-miR-124a총체갑기화솔위73.3%(22/30),명현고우암방조직적10.0%(3/30)(P＜0.05),기갑기화상태여종류대소、분화정도、림파결전이、침범정도등림상병리인소유관(P＜0.01).hsa-miR-124a갑기화여Rb단백화CDK6단백표체지간정정상관(P＜0.05).결론 위암조직중hsa-miR-124a처우고갑기화상태,저충고갑기화가능통과증강Rb급CDK6단백적표체래촉진위암적악성증식.
Objective To investigate the DNA methylation status of the promoter region within the coding gene hsa-miR-124a in human gastric cancer tissue, and examine its association with the expression of Rb and CDK6 protein and clinicopathological factors. Methods Methylation-specific PCR (MS-PCR) was used to detect the DNA methylation status of hsa-miR-124a in gastric cancer tissues and adjacent normal tissues from 30 patients. The expression of Rb and CDK6 protein within these tissues was examined using immunohistochemistry. Results The overall methylation rate of gastric cancer tissues was 73.3%, which was significantly higher than that in the adjacent tissues (10.0%, P＜0.05). The overall positive rates of Rb and CDK6 protein in gastric cancer tissues were 86.7% and 80.0%, respectively. DNA methylation of hsa-miR-124a was positively correlated with the expression of Rb and CDK6 proteins. Significant associations were found between hypermethylation of hsa-miR-124a and tumor size, differentiation, lymphatic metastasis, and invasion depth (P＜0.01).Conclusions Hypermethylation of hsa-miR-124a is present in gastric cancer, and is associated with increased expression of CDK6 and Rb protein. These may be related to the proliferation and development of malignancy in the stomach.