CAJ | 학술논문

目的了解河北省卢龙地区2008 -2009年5岁以下住院儿童中诺如病毒的分子流行病学特征.方法收集2008年10月至2009年8月325例5岁以下腹泻住院患儿粪便标本和流行病学资料,采用酶联免疫吸附试验( ELISA)检测轮状病毒抗原,利用多重RT-PCR方法检测诺如病毒,并对部分诺如病毒阳性株进行序列测定和系统进化分析.结果诺如病毒的检出率为11.3％ (37/325),仅次于轮状病毒的检出率(48.6％),高于腺病毒(6.5％)和星状病毒(4.3％),主要感染2岁以下儿童,季节高峰在11月,系统进化分析表明诺如病毒流行优势株为GⅡ-4/2006b变异株,并发现一株未见报道的新型GⅡ-4变异株.结论诺如病毒是引起2008 -2009年卢龙地区的急性胃肠炎的重要病原之一,GⅡ-4/2006b变异株仍是流行优势株,要进一步监测新型GⅡ-4变异株的流行.
목적 료해하북성로룡지구2008 -2009년5세이하주원인동중낙여병독적분자류행병학특정.방법 수집2008년10월지2009년8월325례5세이하복사주원환인분편표본화류행병학자료,채용매련면역흡부시험( ELISA)검측륜상병독항원,이용다중RT-PCR방법검측낙여병독,병대부분낙여병독양성주진행서렬측정화계통진화분석.결과 낙여병독적검출솔위11.3％ (37/325),부차우륜상병독적검출솔(48.6％),고우선병독(6.5％)화성상병독(4.3％),주요감염2세이하인동,계절고봉재11월,계통진화분석표명낙여병독류행우세주위GⅡ-4/2006b변이주,병발현일주미견보도적신형GⅡ-4변이주.결론 낙여병독시인기2008 -2009년로룡지구적급성위장염적중요병원지일,GⅡ-4/2006b변이주잉시류행우세주,요진일보감측신형GⅡ-4변이주적류행.
Objective To investigate the molecular epidemiologic characteristics and genotypes of norovirus in children less than 5 years of age in Lulong area from 2008 to 2009.Methods 325 stool specimens and epidemiological data from hospitalized children with diarrhea less than 5 years of age were collected.Rotavirus was detected by using the ELISA kit.Norovirus,adenovirus and astrovirus were detected by multiple reverse transcription-polymerase chain reaction (RT-PCR).Partial norovirus strains were sequenced and the tree was conducted by using the phylogenetic analyses.Results Norovirus was detected in 37out of 325 (11.3％ ) specimens,ranked only second to rotavirus (48.6％),and higher than adenovirus (6.5 ％ ) and astrovirus (4.3％).Norovirus predominantly infected children less than 2 years of age and the season peak of norovirus occurred in November.Phylogenetic analysis showed that the predominant strain was the GⅡ.4/2006b variant.Interestingly,a novel unreported GⅡ-4 variant was found in this study.Conclusion Norovirus was one of the most important pathogens causing acute gastroenteritis from 2008 to 2009 in Lulong area.The GⅡ.4/2006b vairant was still the predominant strain.It is important to keep on monitoring the novel GⅡ.4 variant.