CAJ | 학술논문

目的脉冲场凝胶电泳(PFGE)在食源性疾病病原菌诊断中的应用.方法采集19例患者和18名厨师的肛拭子、9份自制冷饮、2份井水以及5例患者的急性期和恢复期血清.同时采集未发病学生大便10份,血清5份,作为正常人对照.用传统的方法进行致病菌的分离和表型鉴定,对所分离的菌株参照美国疾病预防控制中心(CDC)的实验方法用PFGE进行DNA分子分型,使用BioNumerics软件进行聚类分析.方法从19份食物中毒患者肛拭子中分离出14株慕尼黑沙门菌、从9份可疑食物中分离出3株慕尼黑沙门菌、从18名厨师肛拭子中分离出7株慕尼黑沙门菌,所分离菌株生化结果一致、耐药性相同,患者恢复期血清比急性期血清对所分离的菌株抗体有4倍以上增长.23株分离株的PFGE型完全一致.结论PFGE能直观判断肠道致病菌的亲缘关系,及时确定传染源、传播途径和流行范围,是有效控制食源性疾病大面积暴发的早期预警手段.
목적 맥충장응효전영(PFGE)재식원성질병병원균진단중적응용.방법 채집19례환자화18명주사적항식자、9빈자제랭음、2빈정수이급5례환자적급성기화회복기혈청.동시채집미발병학생대편10빈,혈청5빈,작위정상인대조.용전통적방법진행치병균적분리화표형감정,대소분리적균주삼조미국질병예방공제중심(CDC)적실험방법용PFGE진행DNA분자분형,사용BioNumerics연건진행취류분석.방법 종19빈식물중독환자항식자중분리출14주모니흑사문균、종9빈가의식물중분리출3주모니흑사문균、종18명주사항식자중분리출7주모니흑사문균,소분리균주생화결과일치、내약성상동,환자회복기혈청비급성기혈청대소분리적균주항체유4배이상증장.23주분리주적PFGE형완전일치.결론PFGE능직관판단장도치병균적친연관계,급시학정전염원、전파도경화류행범위,시유효공제식원성질병대면적폭발적조기예경수단.
Objective To investigate the application of pulsed-field gel electrophoresis(PFGE) in food-borne outbreak.Methods Pathogens were isolated and further characteristics identified by traditional methods.The strains isolated were carried out with molecular typing with using PFGE.PFGE was performed by Laboratory Directions for molecular subtyping of Salmonella by PFGE ( CDC,USA) and the results of PFGE were analyzed by BioNumerics soft.Results Totally 14 Salmonella serotype Muenchen strains were isolated from 19 patients,3 of 9 suspicious foods were positive for S.muenchen and 7 strains were isolated from 18 cooks.The biochemistry characterization and antimicrobial susceptibility of all the strains isolated were the same.23S.muenchen isolates were all shown indistinguishable by PFGE.Conclusion PFGE should play a key role in identifying the outbreak-associated isolates and distinguishing them from unrelated sporadic isolates.It might also demonstrate that the genetic fingerprints of serotype Muenchen isolates derived from patients were indistinguishable from those derived from drinks.PFGE might provide precise information on bacterial food-borne pathogens,promptly identify the source of infection,and effectively prevent from spreading.It should be one of the early warning method on controlling outbreak of the food-borne disease.