中华预防医学杂志
中華預防醫學雜誌
중화예방의학잡지
CHINESE JOURNAL OF
2010年
1期
34-38
,共5页
魏延%沈新南%麦嘉仪%沈慧%王若仲%吴岷
魏延%瀋新南%麥嘉儀%瀋慧%王若仲%吳岷
위연%침신남%맥가의%침혜%왕약중%오민
番茄红素%活性氧%大鼠%Sprague-Dawley%大脑中动脉栓塞
番茄紅素%活性氧%大鼠%Sprague-Dawley%大腦中動脈栓塞
번가홍소%활성양%대서%Sprague-Dawley%대뇌중동맥전새
Lycopene%Reactive oxygen species%Rats%Sprague-Dawley%Middle cerebral artery occlusion
目的 探讨番茄红素(lycopene,LP)对大鼠大脑中动脉栓塞(middle cerebral artery occlusion,MCAO)所致氧化应激及缺氧损伤的作用及可能机制.方法 48只雄性SD大鼠按体重分层分为A组(20 mg/kg LP)、B组(5 mg/kg LP)、C组(色拉油)、D组(色拉油)及E组(正常饮食).灌胃15 d后,对A、B、C组大鼠制备MCAO模犁,D组行假手术对照.再灌注24 h后测定脑皮质活性氧(reactive oxygen species,ROS)、一氧化氮(NO)、一氧化氮合成酶(NOS)、乳酸(LD)的含量及活性;并采用逆转录-PCR(RT-PCR)法检测大鼠大脑海马组织缺氧诱导因子-1α(hypoxia-inducible factor-1α,HIF-1α)mRNA、Bcl-2 mRNA的表达水平.结果 A、B、C、D、E各组大鼠脑ROS水平分别为(114.23±18.91)、(135.89±14.17)、(171.37±25.76)、(94.24±2.23)、(92.06±5.59)荧光强度值/g蛋白(F=9.038,P<0.01);NO水平分别为(6.60±0.77)、(7.13±0.47)、(8.38±0.80)、(5.52±0.16)、(5.23±0.51)μmol/g蛋白(F=10.197,P<0.01);NOS水平分别为(0.817±0.016)、(0.875±0.095)、(1.030±0.101)、(0.557±0.094)、(0.595±0.066)U/mg蛋白(F=14.555,P<0.01);LD水平分别为(0.381±0.069)、(0.446±0.012)、(0.576±0.059)、(0.359±0.021)、(0.310±0.036)mmol/g蛋白(F=10.043,P<0.01);各组HIF-1α mRNA表达水平分别为0.865±0.274、0.635±0.069、0.491±0.067、0.375±0.052、0.361±0.087(F=40.520,P<0.01);海马Bcl-2 mRNA表达水平分别为0.263±0.033、0.330±0.028、0.198±0.034、0.304±0.039、0.236±0.025(F=11.003,P<0.01).结论 LP对MCAO大鼠脑组织的保护作用可能与其减少缺血冉灌注后ROS和LD的蓄积、减轻炎症反应、上调HIF-1α mRNA、Bcl-2 mRNA的表达水平有关.
目的 探討番茄紅素(lycopene,LP)對大鼠大腦中動脈栓塞(middle cerebral artery occlusion,MCAO)所緻氧化應激及缺氧損傷的作用及可能機製.方法 48隻雄性SD大鼠按體重分層分為A組(20 mg/kg LP)、B組(5 mg/kg LP)、C組(色拉油)、D組(色拉油)及E組(正常飲食).灌胃15 d後,對A、B、C組大鼠製備MCAO模犛,D組行假手術對照.再灌註24 h後測定腦皮質活性氧(reactive oxygen species,ROS)、一氧化氮(NO)、一氧化氮閤成酶(NOS)、乳痠(LD)的含量及活性;併採用逆轉錄-PCR(RT-PCR)法檢測大鼠大腦海馬組織缺氧誘導因子-1α(hypoxia-inducible factor-1α,HIF-1α)mRNA、Bcl-2 mRNA的錶達水平.結果 A、B、C、D、E各組大鼠腦ROS水平分彆為(114.23±18.91)、(135.89±14.17)、(171.37±25.76)、(94.24±2.23)、(92.06±5.59)熒光彊度值/g蛋白(F=9.038,P<0.01);NO水平分彆為(6.60±0.77)、(7.13±0.47)、(8.38±0.80)、(5.52±0.16)、(5.23±0.51)μmol/g蛋白(F=10.197,P<0.01);NOS水平分彆為(0.817±0.016)、(0.875±0.095)、(1.030±0.101)、(0.557±0.094)、(0.595±0.066)U/mg蛋白(F=14.555,P<0.01);LD水平分彆為(0.381±0.069)、(0.446±0.012)、(0.576±0.059)、(0.359±0.021)、(0.310±0.036)mmol/g蛋白(F=10.043,P<0.01);各組HIF-1α mRNA錶達水平分彆為0.865±0.274、0.635±0.069、0.491±0.067、0.375±0.052、0.361±0.087(F=40.520,P<0.01);海馬Bcl-2 mRNA錶達水平分彆為0.263±0.033、0.330±0.028、0.198±0.034、0.304±0.039、0.236±0.025(F=11.003,P<0.01).結論 LP對MCAO大鼠腦組織的保護作用可能與其減少缺血冉灌註後ROS和LD的蓄積、減輕炎癥反應、上調HIF-1α mRNA、Bcl-2 mRNA的錶達水平有關.
목적 탐토번가홍소(lycopene,LP)대대서대뇌중동맥전새(middle cerebral artery occlusion,MCAO)소치양화응격급결양손상적작용급가능궤제.방법 48지웅성SD대서안체중분층분위A조(20 mg/kg LP)、B조(5 mg/kg LP)、C조(색랍유)、D조(색랍유)급E조(정상음식).관위15 d후,대A、B、C조대서제비MCAO모리,D조행가수술대조.재관주24 h후측정뇌피질활성양(reactive oxygen species,ROS)、일양화담(NO)、일양화담합성매(NOS)、유산(LD)적함량급활성;병채용역전록-PCR(RT-PCR)법검측대서대뇌해마조직결양유도인자-1α(hypoxia-inducible factor-1α,HIF-1α)mRNA、Bcl-2 mRNA적표체수평.결과 A、B、C、D、E각조대서뇌ROS수평분별위(114.23±18.91)、(135.89±14.17)、(171.37±25.76)、(94.24±2.23)、(92.06±5.59)형광강도치/g단백(F=9.038,P<0.01);NO수평분별위(6.60±0.77)、(7.13±0.47)、(8.38±0.80)、(5.52±0.16)、(5.23±0.51)μmol/g단백(F=10.197,P<0.01);NOS수평분별위(0.817±0.016)、(0.875±0.095)、(1.030±0.101)、(0.557±0.094)、(0.595±0.066)U/mg단백(F=14.555,P<0.01);LD수평분별위(0.381±0.069)、(0.446±0.012)、(0.576±0.059)、(0.359±0.021)、(0.310±0.036)mmol/g단백(F=10.043,P<0.01);각조HIF-1α mRNA표체수평분별위0.865±0.274、0.635±0.069、0.491±0.067、0.375±0.052、0.361±0.087(F=40.520,P<0.01);해마Bcl-2 mRNA표체수평분별위0.263±0.033、0.330±0.028、0.198±0.034、0.304±0.039、0.236±0.025(F=11.003,P<0.01).결론 LP대MCAO대서뇌조직적보호작용가능여기감소결혈염관주후ROS화LD적축적、감경염증반응、상조HIF-1α mRNA、Bcl-2 mRNA적표체수평유관.
Objective To study the protective effects of lycopene (LP) on cerebral ischemia-reperfusion injury induced by focal cerebral ischemia and oxidative stress in rats. Methods48 male Sprague-Dawley(SD) rats were randomly assigned into five groups:A (20 mg/kg LP) ,B(5 mg/kg LP) ,C(salad oil), D( salad oil)and E (basic feed control). A, B and C groups were given LP or salad oil orally for 15 d, then cerebral ischemia-reperfusion injury was established by middle cerebral artery occlusion (MCAO)and D group was used as fake surgery control. The contents of reactive oxygen species ( ROS), nitric oxide(NO), lactic acid(LD) and the activities of nitric oxide synthetase (NOS) in cortex were measured at 24 h after reperfusion. The levels of HIF-1α mRNA and Bcl-2 mRNA in hippocampi were determined by using reverse transcription polymerase chain reaction (RT- PCR) technique. ResultsROS levels of A, B, C, D and E groups were (114.23 ±18.91),(135.89±14. 17),(171.37±25.76),(94.24±2.23) and (92.06±5.59) fluorescence intensity value/g protein, respectively ( F = 9. 038, P <0.01 ) ; levels of NO were (6. 60±0. 77), (7. 13±0. 47), (8. 38 ±0. 80), (5.52±0. 16) and ( 5. 23±0. 51 )μmol/g protein respectively ( F = 10. 197, P < 0. 01 ) ; levels of NOS were (0. 817±0. 016), (0. 875±0. 095 ), ( 1. 030 ±0. 101 ), (0. 557 ± 0. 094 ) and ( 0. 595 ± 0. 066 ) U/mg protein respectively ( F = 14. 555, P < 0. 01 ) ;levels of LD were (0.381 ± 0.069), (0.446 ± 0.012), (0.576 ±0.059), (0.359±0.021) and (0. 310±0. 036) mmol/g protein respectively ( F = 10. 043 ,P <0. 01 ) ; HIF-1α mRNA expression levels in hippocampi were 0. 865±0. 274,0. 635±0. 069,0. 491±0. 067,0. 375±0. 052 and 0. 361±0. 087,respectively ( F = 40. 520, P < 0. 01 ) ; and Bcl-2 mRNA expression levels in hippocampi were 0. 263±0. 033,0. 330±0. 028,0. 198±0. 034,0. 304±0. 039 and 0. 236±0. 025, respectively ( F = 11. 003, P <0. 01 ). ConclusionThe protective effects of LP may be related with its abilities of decreasing ROS and LD cumulation ,alleviating inflammation and up-regulating the expression of protective genes.
