CAJ | 학술논문

以5个亚洲梨品种为试材,通过离体培养研究了花穗中花的着生部位,不同浓度的GAs、2,4-D和蔗糖对花粉生活力、花粉管生长速度的影响.结果表明,不同品种的花粉生活力差异明显,黄花花粉萌发快;新世纪花粉生活力强,花粉管生长迅速.花的着生位置及花器官的发育程度对花粉生活力有明显影响,边花的生活力比中心花强.不同浓度的B、2,4-D、GA3对花粉萌发及花粉管生长均有不同程度的影响,高浓度的GA3、2,4-D抑制花粉管的生长,但浓度较高的蔗糖能促进花粉粒的萌发和生长,且两者呈正相关(R2=0983).
이5개아주리품충위시재,통과리체배양연구료화수중화적착생부위,불동농도적GAs、2,4-D화자당대화분생활력、화분관생장속도적영향.결과표명,불동품충적화분생활력차이명현,황화화분맹발쾌;신세기화분생활력강,화분관생장신속.화적착생위치급화기관적발육정도대화분생활력유명현영향,변화적생활력비중심화강.불동농도적B、2,4-D、GA3대화분맹발급화분관생장균유불동정도적영향,고농도적GA3、2,4-D억제화분관적생장,단농도교고적자당능촉진화분립적맹발화생장,차량자정정상관(R2=0983).
The pollen viability of 5 cultivars of Asian pear, the influence of flower position in a cluster and the effect of GA3, 2,4-D, B and sucrose on its germination and tube development were studied in vitro. The difference of viability among 5 cultivars was significant. The pollen of Huanghua germinated fast. Both germination rate and pollen tube length of Shinseiki were much higher and longer. Nijisseiki had the poorest performances on germination. Huanqing showed a low capability on pollen tube development; the length was the shortest within the 5 varieties. The flower position in a cluster and developmental stage influenced pollen viability;the viability of pollen grain from outer flowers of a cluster was greater than those from central flowers. Mediate concentration of B,GA3 and 2,4-D increased germination and pollen tube growth. The germination rate and pollen tube length on the medium that contained 15 mg/L of boron were greater than those of other treatments. There were negative relationship trend between pollen viability and the content of GA3 and 2,4-D. High content of sucrose on the medium benefited germination and pollen tube development. There was a linear regression relationship between germination rate and content of sucrose in the medium (R2=0.983).