CAJ | 학술논문

以IR36(indica)和热研2号(japonica,广亲和品种)为亲本,构建了包含180个单株的F2群体及包括110个标记的分子连锁图谱.利用该F2群体,进行了水稻花粉不育数量性状基因座(quantitative trait locus,QTL)的检测和遗传效应分析,共检测到3个花粉不育QTL,分别位于第3、5、7染色体上,此外,共检测到9个由雄配子引起的偏分离QTL,其中7个与ga-14和ga-11位点的配子败育类型相同.与花粉形态鉴定相比,偏分离的数据对检测F1杂种花粉败育基因更为敏感.在第5、6染色体上控制偏分离的2个QTL位点,其杂合基因型出现的频率偏高.在qHPS-5位点,粳型纯合子表现出比杂合子和籼型纯合子更低的育性水平.本研究获得的分子标记将有助于聚合尽可能多的中性亲和基因以解决亚种间F1杂种的花粉不育性问题.
이IR36(indica)화열연2호(japonica,엄친화품충)위친본,구건료포함180개단주적F2군체급포괄110개표기적분자련쇄도보.이용해F2군체,진행료수도화분불육수량성상기인좌(quantitative trait locus,QTL)적검측화유전효응분석,공검측도3개화분불육QTL,분별위우제3、5、7염색체상,차외,공검측도9개유웅배자인기적편분리QTL,기중7개여ga-14화ga-11위점적배자패육류형상동.여화분형태감정상비,편분리적수거대검측F1잡충화분패육기인경위민감.재제5、6염색체상공제편분리적2개QTL위점,기잡합기인형출현적빈솔편고.재qHPS-5위점,갱형순합자표현출비잡합자화선형순합자경저적육성수평.본연구획득적분자표기장유조우취합진가능다적중성친화기인이해결아충간F1잡충적화분불육성문제.
To estimate QTL for the pollen sterility, an F2 population of 180 plants from an indica / japonica hybrid was tested with 110 molecular markers. By the genome wide screening, three QTLs were identified for the pollen sterility on chromosomes 3, 5, 7, respectively. In addition, a total of nine QTL for segregation distortion via male gametes were mapped on rice chromosomes, of which seven QTLs were suggested to be the same type of gamete abortion as found at ga14 and ga-11. The distortion data seemed to be more sensitive to detection of pollen abortion genes in the F1 hybrid than the morphological inspection of pollen. Two QTLs for the segregation distortion on chromosomes 5 and 6 indicated an abnormally high frequency of heterozygous genotype. At a locus indicated by qHPS-5, japonica-type homozygote showed a lower fertility level than the heterozygotes and indica-type homozygote. The molecular markers identified here would be useful in introducing a set of new and known sterility-neutral alleles to solve the pollen sterility problem in subspecific F1 hybrids. Further, the present results could be helpful to combine as many neutral alleles for distortion as possible on whole genome basis to stabilize the pollen fertility as a whole.