南方医科大学学报
南方醫科大學學報
남방의과대학학보
JOURNAL OF SOUTHERN MEDICAL UNIVERSITY
2009年
10期
2107-2108,2111
,共3页
陈谨萍%李海翩%金盛华%张金桃%李军
陳謹萍%李海翩%金盛華%張金桃%李軍
진근평%리해편%금성화%장금도%리군
白癜风%黑素细胞%黑素瘤抗原-1%酶联免疫吸附试验
白癜風%黑素細胞%黑素瘤抗原-1%酶聯免疫吸附試驗
백전풍%흑소세포%흑소류항원-1%매련면역흡부시험
vitiligo%melanocytes%melanoma antigen recognized by T-cells%ELISA
目的 了解儿童白癜风患者血清中抗黑素细胞抗体情况及探讨黑素瘤抗原-1(MART-1)与儿童白癜风的关系.方法 采集患儿血清.检测自身抗体,根据抗体滴度高低分成两组,与正常人黑素细胞进行孵育,利用MART-1特异性抗体检测孵育后黑素细胞表面抗原MART-1的变化.结果 白癜风患儿血清抗黑素细胞抗体滴度升高.与高滴度抗体患儿血清孵育后的MART-1表达明显增加,并能与MART-1特异性抗体丰H结合.结论 部分儿童白癜风是免疫相关的,MART-1与儿童白癜风可能相关联.
目的 瞭解兒童白癜風患者血清中抗黑素細胞抗體情況及探討黑素瘤抗原-1(MART-1)與兒童白癜風的關繫.方法 採集患兒血清.檢測自身抗體,根據抗體滴度高低分成兩組,與正常人黑素細胞進行孵育,利用MART-1特異性抗體檢測孵育後黑素細胞錶麵抗原MART-1的變化.結果 白癜風患兒血清抗黑素細胞抗體滴度升高.與高滴度抗體患兒血清孵育後的MART-1錶達明顯增加,併能與MART-1特異性抗體豐H結閤.結論 部分兒童白癜風是免疫相關的,MART-1與兒童白癜風可能相關聯.
목적 료해인동백전풍환자혈청중항흑소세포항체정황급탐토흑소류항원-1(MART-1)여인동백전풍적관계.방법 채집환인혈청.검측자신항체,근거항체적도고저분성량조,여정상인흑소세포진행부육,이용MART-1특이성항체검측부육후흑소세포표면항원MART-1적변화.결과 백전풍환인혈청항흑소세포항체적도승고.여고적도항체환인혈청부육후적MART-1표체명현증가,병능여MART-1특이성항체봉H결합.결론 부분인동백전풍시면역상관적,MART-1여인동백전풍가능상관련.
Objective To detect the serum levels of melanocyte antibodies and explore the relation between melanoma antigen recognized by T-cells (MART-1) and vitiligo in children. Methods The serum samples were collected from children with vitiligo to test the autoantibodies, and divided into low- and high-titer group according to the test results. Melanocytes were incubated with the serum samples, and the changes of melanocyte surface antigen were evaluated using specific MART-1 antibody. Results The serum melanocyte antibody levels in children with vitiligo were significantly higher than those in normal subjects. The expression level of melanocyte surface antigen MART-1 increased obviously after incubation of the melanocyte with high antibody titer serum samples, and MART-1 was found to specifically bind to specific MART-1 antibody. Conclusion Melanocytes MART-1 may correlate to the autoimmune mechanism in children with vitiligo.