CAJ | 학술논문

采用双酶水解法提取牛蒡菊糖.首先从8种酶中选取3种对牛蒡菊糖提取率最高的酶,分别为木瓜蛋白酶、植物蛋白酶和酸性蛋白酶,对牛蒡菊糖的提取率分别为8.83%、8.67%和8.21%.然后对每一种酶采用单因素试验方法研究pH值、固液比、加酶量、温度以及时间对牛蒡菊糖提取率的影响;再通过3种酶之间的相互组合试验,选出一组最佳组合为:木瓜蛋白酶+植物蛋白酶,其提取率为11.43%.最后采用单因素和正交试验设计方法,研究加酶量、固液比、温度、时间以及pH值对牛蒡菊糖提取率的影响,得到双酶水解提取牛蒡菊糖的最佳条件组合为:在木瓜蛋白酶加酶量10%、温度50℃、pH7、时间4h、固液比1:15(m/V)的条件下进行酶解,4h后加入植物蛋白酶,加酶量20%、温度45℃、pH8,时间4h、固液比1:15(m/V),提取液经乙醇沉淀、真空浓缩,得到粗菊糖,菊糖提取率为13.41%,产品中菊糖含量为67.86%,蛋白质含量为1.32%.
채용쌍매수해법제취우방국당.수선종8충매중선취3충대우방국당제취솔최고적매,분별위목과단백매、식물단백매화산성단백매,대우방국당적제취솔분별위8.83%、8.67%화8.21%.연후대매일충매채용단인소시험방법연구pH치、고액비、가매량、온도이급시간대우방국당제취솔적영향;재통과3충매지간적상호조합시험,선출일조최가조합위:목과단백매+식물단백매,기제취솔위11.43%.최후채용단인소화정교시험설계방법,연구가매량、고액비、온도、시간이급pH치대우방국당제취솔적영향,득도쌍매수해제취우방국당적최가조건조합위:재목과단백매가매량10%、온도50℃、pH7、시간4h、고액비1:15(m/V)적조건하진행매해,4h후가입식물단백매,가매량20%、온도45℃、pH8,시간4h、고액비1:15(m/V),제취액경을순침정、진공농축,득도조국당,국당제취솔위13.41%,산품중국당함량위67.86%,단백질함량위1.32%.
The crude inulin was extracted from burdock by dual enzyme hydrolysis. Three enzymes of papain, plant protease and acid protease with high hydrolysis yields of inulin from burdock were selected in this study. The extraction yields of inulin from burdock were 8.83%, 8.67% and 8.21% by these three enzymes, respectively. A singie-factor experiment for each enzyme was used to investigate the effect of pH, solid/liquid ratio, amount of enzyme, temperature and time on yields of inulin from burdock. The enzyme was intercombined and the optimum combination was papain and plant protease, which presented the yields of 11.43%. Lasyly single-factor experiment and orthogonal experimental design were employed to study the effect of enzyme usage, solid/liquid ratio, temperature, time and pH on extraction efficicency from burdock. The optimum extraction conditions for dual enzyme hydrolysis were 10% papain + 20% plant protease, solid/liquid ratio 1:15, 45 ℃, 8 h of nzymolysis (4 h+ 4 h) and pH 8. The extracts were then precipitated by ethanol, concentrated in vacuum and the crude inulin was acquired. The extraction yield of inulin was 13.41%. The contents of inulin and protein in this product were 67.86% and 1.32%, separately.