西北植物学报
西北植物學報
서북식물학보
ACTA BOTANICA BOREALI-OCCIDENTALIA SINICA
2010年
2期
412-416
,共5页
SC-CO_2%萃取%韭菜籽%油%GC-MS
SC-CO_2%萃取%韭菜籽%油%GC-MS
SC-CO_2%췌취%구채자%유%GC-MS
supercritical carbon dioxide%extraction%Allium tuberosum Rottl.seed%oil%gas chromatography-mass spectrometry
以索氏提取法为对照,采用超临界二氧化碳(SC-CO_2)萃取韭菜籽油,气相色谱-质谱联用技术(GC-MS)对韭菜籽油成分进行分析,NIST 02质谱数据库对其进行分析和鉴定.结果表明,SC-CO_2萃取压力为22.25 MPa、温度为40.40℃条件下萃取86.7 min时,萃取得率为17.52%,共分离鉴定出17种物质,其中,饱和脂肪酸以棕榈酸(6.25%)为主,占脂肪酸总量的 9.05%;不饱和脂肪酸主要是亚油酸(69.71%)和油酸(19.53%),占脂肪酸总量的90.50%.采用索氏提取得率为16.50%,共鉴定出10种物质,饱和脂肪酸以棕榈酸(7.22%)为主,占总脂肪酸量的9.84%;不饱和脂肪酸主要是亚油酸(69.34%)和油酸(20.12%),不饱和脂肪酸占脂肪酸总量的90.16%.另外SC-CO_2萃取韭菜籽油还检出单不饱和脂肪酸7-棕榈烯酸、角鲨烯和β-谷甾醇.
以索氏提取法為對照,採用超臨界二氧化碳(SC-CO_2)萃取韭菜籽油,氣相色譜-質譜聯用技術(GC-MS)對韭菜籽油成分進行分析,NIST 02質譜數據庫對其進行分析和鑒定.結果錶明,SC-CO_2萃取壓力為22.25 MPa、溫度為40.40℃條件下萃取86.7 min時,萃取得率為17.52%,共分離鑒定齣17種物質,其中,飽和脂肪痠以棕櫚痠(6.25%)為主,佔脂肪痠總量的 9.05%;不飽和脂肪痠主要是亞油痠(69.71%)和油痠(19.53%),佔脂肪痠總量的90.50%.採用索氏提取得率為16.50%,共鑒定齣10種物質,飽和脂肪痠以棕櫚痠(7.22%)為主,佔總脂肪痠量的9.84%;不飽和脂肪痠主要是亞油痠(69.34%)和油痠(20.12%),不飽和脂肪痠佔脂肪痠總量的90.16%.另外SC-CO_2萃取韭菜籽油還檢齣單不飽和脂肪痠7-棕櫚烯痠、角鯊烯和β-穀甾醇.
이색씨제취법위대조,채용초림계이양화탄(SC-CO_2)췌취구채자유,기상색보-질보련용기술(GC-MS)대구채자유성분진행분석,NIST 02질보수거고대기진행분석화감정.결과표명,SC-CO_2췌취압력위22.25 MPa、온도위40.40℃조건하췌취86.7 min시,췌취득솔위17.52%,공분리감정출17충물질,기중,포화지방산이종려산(6.25%)위주,점지방산총량적 9.05%;불포화지방산주요시아유산(69.71%)화유산(19.53%),점지방산총량적90.50%.채용색씨제취득솔위16.50%,공감정출10충물질,포화지방산이종려산(7.22%)위주,점총지방산량적9.84%;불포화지방산주요시아유산(69.34%)화유산(20.12%),불포화지방산점지방산총량적90.16%.령외SC-CO_2췌취구채자유환검출단불포화지방산7-종려희산、각사희화β-곡치순.
Supercritical carbon dioxide (SC-CO_2) extract Allium tuberosum Rottl.seeds oil,Soxhlet method extraction as control experiment,gas chromatography-mass spectrometry (GC-MS) were employed to assay the oil chemical components in A.tuberosum Rottl.seed and NIST 02 spectral database were used to analyze and identify these components.SC-CO_2 extraction parameters were:pressure (22.25 MPa),temperature (40.40℃) and time (86.70 min),the value of oil extraction from A.tuberosum Rottl.seeds was 17.52%.17 compositions of seeds oil were identified by GC-MS.The totally fatty acids in the extracts compose of 9.05% saturated fatty acids and 90.50% unsaturated fatty acids.Furthermore,linoleic (69.71%),oleic (19.53%) and palmitic (6.25%) were found the most abundant fatty acids in SC-CO_2 extraction seeds oil.The value of Soxhlet method extraction oil from A.tuberosum Rottl.seeds was 16.50%.10 compositions of seeds oil were identified by GC-MS.Lnoleic (69.34%),oleic (20.12%) and palmitic (7.22%) were found the most abundant fatty acids in seed oil.The totally fatty acids in the Soxhlet method extracts composed of 9.84% saturated fatty acids and 90.16% unsaturated fatty acids.In addition,the monounsaturated fatty acid (7-Hexadecenoic acid),squalene and β-sitosterol were found in SC-CO_2 extraction oil of A.tuberosum Rottl.seeds.
