CAJ | 학술논문

采用单细胞凝胶电泳(SCGE)技术,研究了在不同的染毒时间内不同浓度的五氯酚(PCP)对稀有鮈鲫(Gobiocypris rarus)血细胞和肝脏细胞DNA的损伤.结果显示,各PCP染毒组细胞彗星尾部DNA含量(%DNAT)和彗星尾长(TL)显著增加,与空白对照组比较,差异极显著(P<0.05).随着浓度的增加,细胞彗星尾部%DNAT和TL逐渐增加,其相关系数>0.926,说明在实验浓度范围内,存在显著的浓度-效应关系.在同一浓度下,随着暴露时间的增加,处理组%DNAT和TL逐渐增加,存在显著的时间-效应关系.由于PCP可引起稀有鮈鲫血细胞和肝脏细胞DNA的严重损伤,因此稀有鮈鲫血细胞和肝脏细胞DNA的损伤可作为PCP遗传毒性的指示.
채용단세포응효전영(SCGE)기술,연구료재불동적염독시간내불동농도적오록분(PCP)대희유구즉(Gobiocypris rarus)혈세포화간장세포DNA적손상.결과현시,각PCP염독조세포혜성미부DNA함량(%DNAT)화혜성미장(TL)현저증가,여공백대조조비교,차이겁현저(P<0.05).수착농도적증가,세포혜성미부%DNAT화TL축점증가,기상관계수>0.926,설명재실험농도범위내,존재현저적농도-효응관계.재동일농도하,수착폭로시간적증가,처리조%DNAT화TL축점증가,존재현저적시간-효응관계.유우PCP가인기희유구즉혈세포화간장세포DNA적엄중손상,인차희유구즉혈세포화간장세포DNA적손상가작위PCP유전독성적지시.
By using the alkaline single cell gel-electrophoresis (SCGE), the DNA damage in blood cells and liver cells of Gobiocypris rarus caused by pentachlorophenol at different exposure time (lday, 4days, 7days) with different concentration of pentachlorophenol (40, 100, 160μg/L) was evaluated. Tail DNA percentage (%DNAT) and tail length (TL) of the three tested groups were significantly different from the control group (P< 0.05). Furthermore, the damage intensity of treated groups increased gradually with respect to the increasing of pentachlorophenol. The concentration-effect relationship was significant for the correlation coeffiencient was graeter than 0.926. Under the same concentration, the % DNAT and TL increased gradually with the time of exposure. The time-effect relationship was also significant. This study recommended that it could be a sensitive monitor of aquatic pollution to use the SCGE to detect the DNA damage in fish blood cells and liver cells. It showed that this assay could be applied in the assessment of water pollution and aquatic mutagens.