CAJ | 학술논문

2-酮戊二酸,铁离子和维生素C是氧化戊二酸依赖型加氧酶辅助因子.有的细菌缺少维生素C,因此可能不含维生素C独立体酶,为了确定细菌中是否含有这种酶系,本文首先利用生物学软件ClustalX, PHYLIP, MEME/MAST 和 HMMER在植物和动物体内验证这个酶系的系统发育关系和保守序列,然后利用那些保守序列可以用来检测细菌是否含有这种酶.分析表明细菌中确实含有2-氧化戊二酸依赖型加氧酶酶系.
2-동무이산,철리자화유생소C시양화무이산의뢰형가양매보조인자.유적세균결소유생소C,인차가능불함유생소C독입체매,위료학정세균중시부함유저충매계,본문수선이용생물학연건ClustalX, PHYLIP, MEME/MAST 화 HMMER재식물화동물체내험증저개매계적계통발육관계화보수서렬,연후이용나사보수서렬가이용래검측세균시부함유저충매.분석표명세균중학실함유2-양화무이산의뢰형가양매매계.
The 2-oxoglutarate-dependent dioxygenase (2ODD) family has a character requiring 2-oxoglutarate, iron ion and ascorbate as essential co-factors. The 2-oxoglutarate and iron-binding sites must be conserved in this super family. Bacteria probably lack ascorbate (Vitamin C);hence, they may not have the ascorbate-dependent forms of enzymes. To identify if it exist in bacteria, software predicting measures identified relationship and conserved sequences of this family in plants and animals were used, and then those conserved sequences can be used to determine if bacteria have this family enzyme.