CAJ | 학술논문

目的研究CD4+CD25+调节性T细胞在诱导自发性肝脏免疫耐受中的作用.方法向受体和供体注射抗CD25抗体(PC61)后进行小鼠原位肝脏移植,观测其生存时间.术后20～30 d切取移植肝脏行HE染色,同时观察CD4+CD25+T细胞对CD4+T细胞和CD8+T细胞功能的影响.结果去除受体而不是供体小鼠的CD4+CD25+T细胞可以导致肝移植排斥反应.而且,去除CD4+CD25+T细胞使移植物的白细胞浸润明显增多,组织损伤加重.同时,去除CD4+CD25+T细胞导致CD4+T细胞的增殖活性和CD8+T细胞的细胞毒活性明显增强.结论受体来源的CD4+CD25+调节性T细胞在小鼠肝脏移植免疫耐受诱导中起重要作用.
목적 연구CD4+CD25+조절성T세포재유도자발성간장면역내수중적작용.방법 향수체화공체주사항CD25항체(PC61)후진행소서원위간장이식,관측기생존시간.술후20～30 d절취이식간장행HE염색,동시관찰CD4+CD25+T세포대CD4+T세포화CD8+T세포공능적영향.결과 거제수체이불시공체소서적CD4+CD25+T세포가이도치간이식배척반응.이차,거제CD4+CD25+T세포사이식물적백세포침윤명현증다,조직손상가중.동시,거제CD4+CD25+T세포도치CD4+T세포적증식활성화CD8+T세포적세포독활성명현증강.결론 수체래원적CD4+CD25+조절성T세포재소서간장이식면역내수유도중기중요작용.
Objective To examine the contribution of CD4+ CD25+ regulatory T cells to liver transplant tolerance. Methods After injection of anti-CD25 monoclonal antibody (mAb, PC61), mouse orthotopic liver transplantation was performed and survivals were determined. The paraffin-embedded sections of hepatic allografts were cut and stained with hematoxylin and eosin (HE). Furthermore, the effect of CD4+ CD25+ regulatory T cells on proliferative response of CD4+ T cells and cytotoxicity of CD8+ T cells was examined by depleting these regulatory T cells. Results Depletion of these cells in the recipients but not in the donors before liver transplantation caused rejection. Histological analyses of hepatic allografts with PC61 treatment showed extensive leukocyte infiltration and tissue destruction, whereas those in the control group showed minimal changes. Moreover, elimination of CD4+CD25+ T cells resulted in the enhancement of both proliferative response of CD4+ T cells and cytotoxicity of CD8+ T cells against donor-type alloantigen. Conclusions These results suggest that CD4+CD25+ regulatory T cells were important for tolerance induction to hepatic allografts.